| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,370,338 | G→A | F310F (TTC→TTT) | dcuA ← | C4‑dicarboxylate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,370,338 | 0 | G | A | 91.1% | 501.8 / 23.4 | 214 | F310F (TTC→TTT) | dcuA | C4‑dicarboxylate antiporter |
| Reads supporting (aligned to +/- strand): ref base G (0/19); new base A (195/0); total (195/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.46e-27 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
Only 100 of 214 total aligned reads displayed.
CAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCA > W3110S.gb/4370311‑4370372 | cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:38668/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:1293995/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:778848/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:165246/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:665952/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:1105592/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:1410518/62‑1 (MQ=255)cAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCa < 1:1008358/62‑1 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:600586/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:585859/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:477070/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:572674/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:567763/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:561169/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:533555/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:517654/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:613309/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:507680/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:49161/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:661383/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:473497/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:460017/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:423779/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:408259/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:381878/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:374582/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:364140/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:352986/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:344061/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:807223/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:9970/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:981965/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:974310/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:957834/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:94842/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:940801/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:928033/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:891175/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:878603/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:84198/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:836292/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:651236/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:801721/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:784631/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:782260/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:780234/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:766681/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:759440/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:727212/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:704870/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:700699/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:278676/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:123812/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:333408/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1376570/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1366276/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1356468/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1342505/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1330830/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1320776/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1312251/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1306692/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:126472/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1249380/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1380982/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:123420/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1217171/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1209739/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1200030/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1187726/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1174204/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1167938/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1132455/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1079442/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1056218/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1020117/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1594281/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:296513/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:269101/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:259341/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:252257/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:235105/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:200085/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:191711/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1635023/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1604021/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1402461/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1571256/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1564654/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1560189/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:154076/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1527896/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:151648/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1496803/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1492775/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1452249/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:142740/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAcag > 1:1411889/1‑37 (MQ=255) aTGAACAGACCAGACACCGCAGCAAAAGAAGCAAaag > 1:874681/1‑37 (MQ=38) aTGAACAGACCAGACACCGCAGAAAAAGAAGCAAcag > 1:218602/1‑37 (MQ=37) | CAGAATGAACAGACCAGACACCGCAGCGAAAGAAGCAACAGCGGTCAGCGGTGAAACGTTCA > W3110S.gb/4370311‑4370372 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |