Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,438,386 T→G A107A (GCA→GCC)  ytfG ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,438,3860TG99.6% 723.7 / NA 249A107A (GCA→GCC) ytfGNAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (0/248);  total (0/249)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

GGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGC  >  W3110S.gb/4438362‑4438399
                        |             
ggCGAGGCCGATCGGGGAGGAATCGGCATGTAGCAGGc  <  1:301413/38‑1 (MQ=37)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:463211/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:68188/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:672990/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:66737/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:665034/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:644007/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:63063/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:620091/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:600197/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:57347/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:547787/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:50675/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:70792/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:457196/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:41240/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:404605/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:382664/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:373250/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:337198/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:32449/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1001314/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:309546/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:285462/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:81161/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:984091/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:980846/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:943384/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:934499/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:92608/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:916356/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:903304/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:869966/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:845619/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:83261/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:826952/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:317360/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:80091/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:794400/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:775006/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:755457/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:747947/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:737683/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:731444/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:714740/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:713981/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:709535/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1236482/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1509650/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1479405/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1441062/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1426722/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1416964/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1398494/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1366817/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1342294/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1307926/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:128810/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:126084/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:262650/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1156555/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:113880/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1122362/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1114800/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1085556/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1075789/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1051394/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:104906/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1039863/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:102047/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:272950/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1544991/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:260495/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:222352/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:206447/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:199944/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:185564/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1578990/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:167705/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1638050/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1607266/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1618968/38‑1 (MQ=38)
ggCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1514096/38‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCTGCATGTAGCAGGc  <  1:877365/37‑1 (MQ=255)
 gCGAGGCCGAGCGGGGAGGAATCGGGATGTAGCAGGc  <  1:416315/37‑1 (MQ=37)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:425300/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1193215/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1192759/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1263462/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1597117/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1335076/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:51248/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:533883/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:350055/37‑1 (MQ=38)
 gCGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1571873/37‑1 (MQ=38)
  cGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:559951/36‑1 (MQ=38)
  cGAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1549780/36‑1 (MQ=38)
   gAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1180755/35‑1 (MQ=38)
   gAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1451459/35‑1 (MQ=38)
   gAGGCCGAGCGGGGAGGAATCGGCATGTAGCAGGc  <  1:1491029/35‑1 (MQ=38)
                        |             
GGCGAGGCCGAGCGGGGAGGTATCTGCATGTAGCAGGC  >  W3110S.gb/4438362‑4438399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑