| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,461,168 | C→T | C234C (TGC→TGT) | mpl → | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,461,168 | 0 | C | T | 87.8% | 533.7 / 44.3 | 245 | C234C (TGC→TGT) | mpl | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base C (26/4); new base T (215/0); total (241/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 245 total aligned reads displayed.
TTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG > W3110S.gb/4461116‑4461222 | ttatCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagc < 1:1030593/59‑1 (MQ=255)ttatCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagc < 1:1032422/59‑1 (MQ=255)ttatCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagc < 1:1179036/59‑1 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:720693/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:329991/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:296311/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:626579/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:631881/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:1537017/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:1191545/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:937551/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:106157/1‑61 (MQ=255) atCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGgagcgagc > 1:970946/1‑61 (MQ=255) tGGTCTGTTGGAGCGAGCTGGAGCTGGTGGGCGAGCAAGGt > 1:282300/1‑41 (MQ=16) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGaaaaa > 1:1571102/1‑58 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAg > 1:806425/1‑59 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt > 1:1014626/1‑61 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt > 1:67672/1‑61 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt > 1:1429066/1‑61 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:482088/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:964993/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:624017/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:595150/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:577904/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:567169/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:550828/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:5295/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:509221/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:359066/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:475387/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:457645/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:4241/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:398140/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:36780/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:926761/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:793232/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:923816/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:896467/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:946531/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:865522/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:863376/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:856867/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:831486/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:827476/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:820379/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:797754/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:648529/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:778346/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:734889/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:725337/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:980308/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:715598/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:70842/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:699288/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:692322/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:960303/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1216627/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1451902/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1408838/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1370030/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:132810/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1314122/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1303413/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1262201/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1253974/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1243155/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1233371/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1458673/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1208320/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1168132/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1146978/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1136213/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1121305/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1111769/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1104269/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1087783/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1071822/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1055900/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1626298/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:316225/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:272342/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:261452/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:232200/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:227427/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:206206/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:197166/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1647643/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1633039/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:33984/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1623553/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1614239/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1607811/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1594273/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1553582/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1520582/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1492866/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:1486567/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg > 1:147709/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGACACTGGCAGGCGAAAAAGCTg > 1:1528355/1‑62 (MQ=255) tGGTCTGTTGGAGCGAGAAGGAGCTGGTGGGCGAGCAAGGTAACTGGCAGGCGAAAAAGCTg > 1:179872/1‑62 (MQ=255) | TTATCTGGCCGGAAAATGACATCAACCTGAAACAGACCATGGCGATGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG > W3110S.gb/4461116‑4461222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |