Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,461,192 T→C G242G (GGT→GGC)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,461,1920TC100.0% 630.5 / NA 215G242G (GGT→GGC) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (215/0);  total (215/0)

Only 100 of 215 total aligned reads displayed.

TGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG  >  W3110S.gb/4461161‑4461222
                               |                              
tGGTCTGTTGGAGCGAGCTGGAGCTGGTGGGCGAGCAAGGt                       >  1:282300/1‑41 (MQ=16)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGCGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:542201/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGc                            >  1:1145424/1‑36 (MQ=25)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAg     >  1:806425/1‑59 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:1014626/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:1429066/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:572520/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:825447/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:648529/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:699288/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:633747/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:62552/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:611689/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:595150/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:567169/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:5295/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:509221/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:48652/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:481479/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:45767/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:445059/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:408279/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:379536/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:36780/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:359066/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:355002/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:330479/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:316225/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:856867/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:971168/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:965186/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:964811/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:95615/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:946531/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:935694/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:925718/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:913150/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:896467/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:864087/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:861743/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:677577/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:828359/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:813372/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:797754/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:793232/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:770529/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:734589/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:718994/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:715598/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:702688/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:272342/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:692322/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1180632/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:291447/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1408838/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1370030/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:132810/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1320621/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:130601/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1262201/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1257648/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1243577/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1241966/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1216698/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1451902/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1168132/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1163067/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1129600/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1115214/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1111769/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:109826/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1087783/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1071822/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1061903/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1055900/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1034404/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1607811/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:261452/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:232200/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:227427/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:206206/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:197166/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1647643/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:163958/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1630663/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1623553/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1614239/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1462012/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1595212/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1575432/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1568619/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1553582/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1529919/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1525432/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1518927/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1492866/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1486567/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:147709/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGATg  >  1:1211732/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGAAGGAGCTGGTGGGCGAGCAAGGTAACTGGCAGGCGAAAAAGCTg  >  1:179872/1‑62 (MQ=255)
                               |                              
TGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG  >  W3110S.gb/4461161‑4461222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑