Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 511,674 C→T V270V (GTC→GTT)  ybaS → predicted glutaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb511,6740CT100.0% 993.7 / NA 342V270V (GTC→GTT) ybaSpredicted glutaminase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (342/0);  total (342/0)

Only 100 of 342 total aligned reads displayed.

GCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATG  >  W3110S.gb/511637‑511723
                                     |                                                 
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCGTGGCGTGGTggg                                     >  1:1513975/1‑52 (MQ=37)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:608598/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1252227/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:218890/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:74379/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:210008/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:194901/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:56542/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1344104/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1575155/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:478751/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1410489/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:67356/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1445181/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:628794/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1490916/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1495192/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:649212/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:424738/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:988473/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:982164/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:380239/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:955411/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:943042/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:366994/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1077708/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:914677/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:335392/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:847926/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:82251/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:311163/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1178278/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:469453/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:267217/1‑52 (MQ=38)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGtttt                       >  1:397919/1‑38 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGtttt                       >  1:966514/1‑38 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTtttc                      >  1:69289/1‑41 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGaa      >  1:1002373/1‑57 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:759027/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:290179/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:417568/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:925103/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:896783/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:453691/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:860907/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:810479/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:79965/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:791708/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:766308/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:65889/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:732768/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:731254/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:489502/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:500091/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:534677/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:704937/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:573915/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:601546/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:688081/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:66919/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1123106/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1328372/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:131408/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1304419/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1276436/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1230480/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:122303/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1207628/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1172174/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1148547/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1340262/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1112431/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1104008/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1088911/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1069246/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1055103/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1041089/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1031562/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1025087/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1018252/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1593132/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:351108/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:343661/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:30886/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:249457/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:182715/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:171090/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1648830/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1625993/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1614745/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:387315/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1563561/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1558848/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1542999/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1522529/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1460642/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1423280/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1365862/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1357746/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaa   >  1:631209/1‑59 (MQ=255)
                                     |                                                 
GCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATG  >  W3110S.gb/511637‑511723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑