Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,461,198 G→A Q244Q (CAG→CAA)  mpl → UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,461,1980GA100.0% 615.0 / NA 214Q244Q (CAG→CAA) mplUDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (214/0);  total (214/0)

Only 100 of 214 total aligned reads displayed.

TGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG  >  W3110S.gb/4461161‑4461222
                                     |                        
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCTAAAAAGCTg  >  1:200696/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAg     >  1:806425/1‑59 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:1014626/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:1447526/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCt   >  1:572520/1‑61 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:481479/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:675686/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:646067/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:62552/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:611689/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:595150/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:567169/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:550828/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:534736/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:509221/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:48652/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:692322/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:45767/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:445059/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:4241/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:398140/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:36780/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:359066/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:355002/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:330479/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:320947/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:311670/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:828359/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:971168/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:965186/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:964811/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:95615/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:946531/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:935694/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:926761/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:913150/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:896467/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:864087/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:861743/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:856867/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:677577/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:827476/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:813372/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:797754/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:793232/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:770529/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:734589/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:725127/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:715598/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:702688/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:699288/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:281066/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1172981/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:288814/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1404354/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1351531/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1320621/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:130601/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:127677/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1262069/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1253974/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1243155/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1233371/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1208320/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1425514/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1167907/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1146978/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1129600/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1115214/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1105621/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:109826/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1087783/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1071822/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1061903/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1055900/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1034404/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1598139/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:261452/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:232200/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:227427/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:214627/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:191938/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:179787/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:163958/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1630663/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1623553/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1616203/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1614177/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1457512/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1594273/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1568619/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1553582/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1520582/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1511405/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1487236/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:147709/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1462012/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGATg  >  1:1211732/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGTGGGCGAGCAAGGACACTGGCAGGCGAAAAAGCTg  >  1:1528355/1‑62 (MQ=255)
tGGTCTGTTGGAGCGAGCAGGAGCTGGAGGGCGAGCAAGGTCACTGGCAGGCGAAAAAGCTg  >  1:1531301/1‑62 (MQ=255)
                                     |                        
TGGGCTGCTGGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCGAAAAAGCTG  >  W3110S.gb/4461161‑4461222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑