Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,463,519 G→A E293E (GAG→GAA)  pmbA → predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,463,5190GA100.0% 408.3 / NA 142E293E (GAG→GAA) pmbApredicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (0/142);  total (0/142)

Only 100 of 142 total aligned reads displayed.

AAACAAATTCTGCCGGACTGGCTGACCATTGAAGAGCATCCGCATCTGCTGAAAGGGCTGGC  >  W3110S.gb/4463484‑4463545
                                   |                          
acaCAAATTCTTCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:63443/60‑1 (MQ=255)
acaCAAATTCTGCCTGACTGGCTGACAATTGAAGAACATCCTCATCTGCTGAAAGGGCTGGc  <  1:1044973/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:533441/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:669185/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:658118/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:655045/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:653730/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:6526/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:650215/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:62519/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:618284/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:550009/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:542007/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:53773/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:671805/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:528527/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:476607/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:46459/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:437567/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:429133/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:422322/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:372508/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:372128/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1012070/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:302976/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:867767/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:996028/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:987613/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:974182/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:957923/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:948428/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:946785/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:940056/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:92937/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:926149/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:917476/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:90447/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:326569/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:85585/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:848779/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:784306/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:777466/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:761346/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:758712/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:727142/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:718370/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:688111/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:679033/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:120512/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1537790/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1448252/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1413312/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1393459/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1335282/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1331491/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1303850/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1283187/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1277263/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1233212/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1219511/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1468560/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1179214/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1161650/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1159507/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:113997/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1138266/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1125401/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1113372/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1084595/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1034302/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1023002/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:101967/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:172212/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:261879/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:249835/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:246120/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:233730/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:223306/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:212368/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:211802/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:205518/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:185105/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1647522/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1599157/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1557488/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1562169/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1587075/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1597486/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1639952/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1634271/60‑1 (MQ=255)
acaCAAATTCTGCCGGACTGGCCGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1270240/60‑1 (MQ=255)
 caCAAATTCTTCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:398837/60‑1 (MQ=255)
 caCAAATTCTGCCGGACTGGCTGTCAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:40324/60‑1 (MQ=255)
 caCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:454746/60‑1 (MQ=255)
 caCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1616363/60‑1 (MQ=255)
 caCAAATTCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1398341/60‑1 (MQ=255)
       ttCTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:1543011/55‑1 (MQ=255)
         cTGCCGGACTGGCTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:736954/53‑1 (MQ=39)
                     cTGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:442052/41‑1 (MQ=38)
                      tGACAATTGAAGAACATCCGCATCTGCTGAAAGGGCTGGc  <  1:27927/40‑1 (MQ=37)
                                   |                          
AAACAAATTCTGCCGGACTGGCTGACCATTGAAGAGCATCCGCATCTGCTGAAAGGGCTGGC  >  W3110S.gb/4463484‑4463545

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑