Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	75.4 / NA	28	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (0/28);  total (0/28)

AGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGG  >  W3110S.gb/4563565‑4563626                         |                                      agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:314552/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:8897/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:843936/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:773121/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:627207/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:51079/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:484692/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:381811/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:355311/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:324896/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:203924/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:183198/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1563297/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1378738/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1365478/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1274310/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1273076/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1215537/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1191602/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1143400/62‑1 (MQ=255) agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1026849/62‑1 (MQ=255) agGGCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:462997/62‑1 (MQ=255)  ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1399517/61‑1 (MQ=255)  ggTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:852452/61‑1 (MQ=255)   gTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1008541/60‑1 (MQ=255)      aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:669841/57‑1 (MQ=255)                tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:1531837/47‑1 (MQ=255)                 gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg  <  1:400243/46‑1 (MQ=255)                         |                                      AGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGG  >  W3110S.gb/4563565‑4563626

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑