Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 511,680 A→C G272G (GGA→GGC)  ybaS → predicted glutaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb511,6800AC100.0% 998.7 / NA 341G272G (GGA→GGC) ybaSpredicted glutaminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (341/0);  total (341/0)

Only 100 of 341 total aligned reads displayed.

GCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATG  >  W3110S.gb/511637‑511723
                                           |                                           
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1618266/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1328993/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1344104/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1445181/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1463035/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1490916/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:67356/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:154800/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:649212/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1582659/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:628794/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:988473/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:614998/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1649118/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:202138/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:210008/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:218989/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:56542/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:335392/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:366994/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:469453/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:400870/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:955411/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:914677/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1075164/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:943042/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:131441/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:847926/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:82251/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:1225024/1‑52 (MQ=38)
gCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTTCCTGGCGTGATggg                                     >  1:982164/1‑52 (MQ=38)
                          cTGGCTGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:320530/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGtttt                       >  1:966514/1‑38 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGt                          >  1:1500039/1‑37 (MQ=38)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTtttc                      >  1:69289/1‑41 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTtttc                      >  1:1361942/1‑41 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGaa      >  1:1002373/1‑57 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:484721/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:489502/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:860907/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:506268/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:534677/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:453691/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:290179/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:925103/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:429766/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:577264/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:601546/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:896783/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:812052/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:79965/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:65889/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:791708/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:670688/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:766308/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:759027/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:75400/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:688081/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:704937/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:732768/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:731254/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1172514/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:417568/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1340510/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1307247/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1283016/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1254213/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1230480/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1213343/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1181731/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1377065/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1148547/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1123858/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1115070/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:110529/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1081160/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1045312/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1025198/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1018252/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:185522/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:387315/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:381702/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:351108/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:344495/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:30886/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:268966/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:249457/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1416999/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:171090/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1625993/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1593132/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1572374/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1558848/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1522529/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1515662/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaag  >  1:1425085/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaa   >  1:631209/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGaa   >  1:1094928/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACCACCGCTGGACGAAGa    >  1:1055104/1‑59 (MQ=255)
                          cTGGCGGTCGTTCCTGGCGTGATGGGAATTGCCGCGTTTTCACAACCGCTGGACGAAGaag  >  1:103350/1‑59 (MQ=255)
                                           |                                           
GCAAAAGCGGTGTAGGTGGCGGTATTCTGGCGGTCGTCCCTGGAGTGATGGGAATTGCCGCGTTCTCACCACCGCTGGACGAAGATG  >  W3110S.gb/511637‑511723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑