Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 4,588,402 C→T L1032L (CTG→CTA)  hsdR ← endonuclease R

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,588,4020CT100.0% 359.9 / NA 125L1032L (CTG→CTA) hsdRendonuclease R
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (125/0);  total (125/0)

Only 100 of 125 total aligned reads displayed.

GCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGT  >  W3110S.gb/4588351‑4588442
                                                   |                                        
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCTGGGAACGTTGCACTAGCGAGTCaa                                >  1:349218/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAg                                    >  1:1072396/1‑58 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:391056/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:1064784/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:276812/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:962132/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:193633/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:1575310/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:1258845/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:159460/1‑62 (MQ=255)
gCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCaa                                >  1:80453/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGGAATCCTGCGGCGTGTCGt  >  1:1276396/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcgg          >  1:1417133/1‑54 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCg   >  1:759302/1‑61 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCg   >  1:636386/1‑61 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCg   >  1:93680/1‑61 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCg   >  1:104445/1‑61 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:247948/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:343084/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:494447/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:467861/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:462760/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:442890/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:27019/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:437211/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:396084/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:390783/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:384455/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:28795/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:338706/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:338965/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:6186/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:747692/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1002087/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:964690/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:963787/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:945819/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:903350/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:872306/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:827144/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:820655/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:802312/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:755808/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:520703/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:737235/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:699842/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:685117/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:658320/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:646129/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:97689/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:617537/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:589560/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:545615/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:531867/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:124441/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:242746/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1332609/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1324377/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1323892/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1322160/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:131411/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1311831/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1310102/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1301040/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1257308/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1246276/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1365402/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1233505/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1230863/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:121823/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1213189/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:118229/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1151577/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1123628/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1095226/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1041533/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1022457/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1577203/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:239221/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:217945/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:211819/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:173303/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1647953/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1629203/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1621774/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1619090/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1579876/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1396900/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1566496/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1519954/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1500792/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1500028/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1486023/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1467004/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1459831/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1408512/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:140159/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt  >  1:1398768/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTCTCGt  >  1:1216674/1‑62 (MQ=255)
                              cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCAGCGGCGTGTCGt  >  1:271147/1‑62 (MQ=255)
                                                   |                                        
GCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGT  >  W3110S.gb/4588351‑4588442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑