Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	4,599,743	G→T	T264N (ACC→AAC)	yjjM ←	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,599,743	0	G	T	100.0%	130.6 / NA	47	T264N (ACC→AAC)	yjjM	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (47/0);  total (47/0)

CTGCGGCAAATCAGAGCGCTGAGAATGGTCATATGCTCATCGACGGCAATGATATTGCGCTG  >  W3110S.gb/4599716‑4599777                            |                                   cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACg                   >  1:398763/1‑45 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTgc      >  1:1367030/1‑58 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGctct   >  1:1595610/1‑61 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCt   >  1:219465/1‑61 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:736609/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:426808/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:442884/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:454993/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:475974/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:555645/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:60755/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:645326/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:712097/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:732436/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:308019/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:784633/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:846508/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:848335/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:897636/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:898437/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:964603/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:967661/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:98572/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:991995/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:995479/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:340453/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1242102/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1360805/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1398566/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1400029/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1418080/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:14575/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1463792/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1487891/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1498525/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1521366/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1595574/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:161178/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1629894/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:22055/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:228750/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:23959/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:240156/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:282736/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1002262/1‑62 (MQ=255) cTGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:366631/1‑62 (MQ=255)  tGCGGCAAATCAGAGCGCTGAGAATGTTCATATGCTCATCGACGGCAATGATATTGCGCTg  >  1:1586533/1‑61 (MQ=255)                            |                                   CTGCGGCAAATCAGAGCGCTGAGAATGGTCATATGCTCATCGACGGCAATGATATTGCGCTG  >  W3110S.gb/4599716‑4599777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑