Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 10,703 C→T T218T (ACG→ACA)  yaaW ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb10,7030CT100.0% 685.1 / NA 238T218T (ACG→ACA) yaaWconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (0/238);  total (0/238)

Only 100 of 238 total aligned reads displayed.

ACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCA  >  W3110S.gb/10647‑10707
                                                        |    
acatGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:406618/58‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCTGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:980874/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:620367/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:260456/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:604965/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:58539/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:555388/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:547156/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:535696/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:529815/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:508495/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:482601/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:440751/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:414458/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:39250/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:374968/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:360820/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:347370/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:334561/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:3294/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:320064/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:301874/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:291124/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1014905/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:271911/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:83755/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:975537/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:951790/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:943119/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:93367/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:930078/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:928185/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:912179/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:895455/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:886756/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:876424/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:859951/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:636699/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:824184/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:807970/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:803576/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:792762/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:780412/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:759317/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:740990/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:697132/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:685463/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:660761/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1228733/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1454412/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:144184/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:256941/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1401150/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1388123/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1356485/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1335797/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1326753/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1306608/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1275246/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1239370/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1411976/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:121649/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1196554/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1191874/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1167910/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1124222/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1112021/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1103124/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1040419/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1036993/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1023959/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1019657/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1571665/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:245368/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:230309/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:202525/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:181810/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:179362/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1631861/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1623458/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1619289/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1600045/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1582082/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1547192/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1539490/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1528971/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:15217/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1501006/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1492595/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1477033/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1465718/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAATGTCa  <  1:1377397/61‑1 (MQ=255)
aCCTGAGTGGCGCTAACCACCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1607505/61‑1 (MQ=255)
 ccTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1246141/60‑1 (MQ=255)
 ccTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:862991/60‑1 (MQ=255)
 ccTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1361565/60‑1 (MQ=255)
  cTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:561473/59‑1 (MQ=255)
       tGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:38758/54‑1 (MQ=255)
                    ccGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:464313/41‑1 (MQ=255)
                      ggCGCAGGCAGGCGATTTGCAGTACGGCTGGAATTGTCa  <  1:1294455/39‑1 (MQ=255)
                                                        |    
ACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCA  >  W3110S.gb/10647‑10707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑