Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 530,879 C→T A189A (GCG→GCA)  ybbS ← transcriptional activator of the allD operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb530,8790CT100.0% 476.1 / NA 165A189A (GCG→GCA) ybbStranscriptional activator of the allD operon
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (165/0);  total (165/0)

Only 100 of 165 total aligned reads displayed.

GGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGC  >  W3110S.gb/530862‑530923
                 |                                            
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAAc                       >  1:979610/1‑41 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGtt                    >  1:247618/1‑44 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGcc                >  1:692090/1‑48 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGcc                >  1:537804/1‑48 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCgg           >  1:1031488/1‑53 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:623073/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:251327/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:616418/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:603108/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:600280/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:568279/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:562583/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:557161/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:551437/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:525458/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:493051/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:459669/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:4460/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:414156/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:383158/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:379298/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:367792/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:350895/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:350412/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:345726/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:31158/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:952315/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:801750/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:945395/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:889996/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:874368/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:864289/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:855381/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:841652/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:836501/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:828062/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:825493/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:823802/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:628896/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:778255/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:76204/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:754384/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:745709/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:743648/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:728765/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:698577/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:651087/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:649275/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:641967/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1276274/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1453917/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:248587/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1419747/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1379835/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1358647/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1348862/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1328503/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1320351/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1307321/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1299520/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1283546/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1438792/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1273108/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1241497/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1198780/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1193720/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1170440/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1137465/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1114321/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1100358/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1065684/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1026810/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1011280/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1479861/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:246374/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:212280/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:187611/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:175060/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1627369/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:161574/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1595467/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1575996/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1569729/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1547729/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1533661/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1454523/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1532238/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1522930/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:151600/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1510865/1‑62 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:478254/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:1008237/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:1084899/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:217916/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:1134364/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:165540/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:434368/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccg   >  1:1289369/1‑61 (MQ=255)
ggAAAGCGCCGCAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATc     >  1:273876/1‑59 (MQ=255)
ggAAAGCGCC‑CAACTGTGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATccgc  >  1:1365328/1‑61 (MQ=255)
                 |                                            
GGAAAGCGCCGCAACTGCGCTTCTGTTAGCGGCTCTTCAACGTTCGCCAGCGGATGATCCGC  >  W3110S.gb/530862‑530923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑