| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 565,667 | T→C | intergenic (‑83/‑7) | ybcC ← / → ybcD | predicted exonuclease/DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 565,667 | 0 | T | C | 92.0% | 430.3 / 14.5 | 176 | intergenic (‑83/‑7) | ybcC/ybcD | predicted exonuclease/DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment |
| Reads supporting (aligned to +/- strand): ref base T (14/0); new base C (162/0); total (176/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 178 total aligned reads displayed.
AGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCC > W3110S.gb/565615‑565706 | aGCGAGGCCTGTATCCGGGTGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:873039/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCactact > 1:363820/1‑57 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:347991/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:568163/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:530664/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:514246/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:502974/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:484764/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:436200/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:430269/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:384999/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:376644/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:355952/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:592520/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:342834/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:33133/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:313521/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:2674/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:260790/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:257677/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:251273/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:231835/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1027166/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:165571/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:789475/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:996451/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:985674/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:943058/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:919474/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:897026/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:883481/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:867915/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:866808/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:857095/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:820515/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:813702/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:581033/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:777625/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:734610/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:729226/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:711242/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:689964/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:671610/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:669781/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:664584/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:617431/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:216329/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1190916/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1351620/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1634292/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1304142/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1301926/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1291172/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1277847/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:125086/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1244618/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1236337/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1216600/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1191887/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1337077/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:118449/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1167647/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1139271/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1129567/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1109884/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1107963/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1066723/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1065257/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1054499/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1042663/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1033419/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1355878/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1609908/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:160978/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1596194/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1576426/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:154363/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1535088/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1533782/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1514818/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1502048/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1497957/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1487021/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1440245/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1360439/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1377978/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1382084/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1385644/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1403627/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1405781/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:1423041/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:145006/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTGCAAATAGAACGCGCACTACtggctg > 1:281551/1‑62 (MQ=255)aGCGAGGCCTGTATCCGGATGCAGAGTCTTATCCGTCGAAATCGAACGCGCactact > 1:1039766/1‑57 (MQ=255)aGCGAGGCCTGTATCCGGATCCAGAGTCTTATCCGTGGAAATCGAACGCGCACTACtggctg > 1:975619/1‑62 (MQ=255) gAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc > 1:85340/1‑55 (MQ=255) gAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc > 1:1240567/1‑55 (MQ=255) gAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc > 1:807666/1‑55 (MQ=255) gAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc > 1:196755/1‑55 (MQ=255) gAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGcc > 1:545987/1‑55 (MQ=255) | AGCGTGGCCTGTATCCGGATGCAGAGTCTTATCCGTGGAAATCGAACGCGCATTACTGGTTGGTTACCAACTTGTACCAGAACATGCGGGCC > W3110S.gb/565615‑565706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |