| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 26,534 | T→C | S86S (AGT→AGC) | ispH → | 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate reductase, 4Fe‑4S protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 26,534 | 0 | T | C | 99.6% | 658.0 / ‑4.0 | 227 | coding (258/951 nt) | ispH | 1‑hydroxy‑2‑methyl‑2‑(E)‑butenyl 4‑diphosphate reductase, 4Fe‑4S protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (226/0); minor base . (1/0); total (227/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01 | |||||||||||
Only 100 of 227 total aligned reads displayed.
ATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGG > W3110S.gb/26484‑26547 | aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGAtt > 1:776229/1‑58 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTg > 1:152894/1‑60 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:252478/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:1622754/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:1628966/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:521706/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:457359/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:1277349/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:224174/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:243650/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:111622/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:108747/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGa > 1:365188/1‑61 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:620215/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:543410/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:540053/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:552187/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:527548/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:596141/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:616874/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:683316/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:498229/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:476495/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:443940/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:412808/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:398207/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:37097/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:364030/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:355599/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:346094/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:838561/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:991425/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:987864/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:959780/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:940834/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:923780/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:918548/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:909447/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:898649/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:886361/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:884546/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:876607/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:852870/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:642373/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:829668/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:818712/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:816892/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:805010/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:782305/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:763851/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:747783/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:723440/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:690808/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:282309/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:670426/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1257746/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1390704/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1382434/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1376497/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1355432/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1343241/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1336005/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1331310/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1327640/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1327108/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1321515/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:130783/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1405905/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1240054/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:121084/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:120603/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1197291/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1190736/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1075136/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1072373/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1035524/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1029172/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1015443/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1650306/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:316831/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:31282/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:294803/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1003222/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:276140/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:266231/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:230991/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:206470/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:198885/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:197449/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1597299/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1581919/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1574448/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1539942/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1519313/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1509064/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1491472/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1480561/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:143145/1‑62 (MQ=255)aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:1412690/1‑62 (MQ=255)aTTTTCTACGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGAc > 1:335616/1‑62 (MQ=255) | ATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGG > W3110S.gb/26484‑26547 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |