Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	620,339	A→G	W25R (TGG→CGG)	fepG ←	iron‑enterobactin transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	620,339	0	A	G	100.0%	114.3 / NA	41	W25R (TGG→CGG)	fepG	iron‑enterobactin transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (0/41);  total (0/41)

TTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCA  >  W3110S.gb/620307‑620368                                 |                              ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:533298/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1001927/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1591710/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1598767/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1606798/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:164107/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:182149/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:254407/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:270361/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:425448/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1444515/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:535216/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:547033/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:547138/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:590595/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:611920/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:676318/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:834520/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:866573/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:891923/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1186699/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1009152/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1037419/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1060207/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1107106/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1120598/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1127229/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1143106/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1170943/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1348836/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1190405/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1194524/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1251280/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1255736/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:127413/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1328631/62‑1 (MQ=255) ttCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1341167/62‑1 (MQ=255)  tCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1111567/61‑1 (MQ=255)  tCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:684221/61‑1 (MQ=255)      gCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:392264/57‑1 (MQ=255)                   gCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca  <  1:1308788/44‑1 (MQ=255)                                 |                              TTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCA  >  W3110S.gb/620307‑620368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑