Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	633,239	T→A	L144Q (CTG→CAG)	ybdL →	methionine aminotransferase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	633,239	0	T	A	100.0%	251.6 / NA	90	L144Q (CTG→CAG)	ybdL	methionine aminotransferase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (90/0);  total (90/0)

CGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACTGCAACCACCGCATTTTCGCG  >  W3110S.gb/633198‑633259                                          |                     cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATTGCACAGCAACCACCGCATTTTcgcg  >  1:24477/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGc                    >  1:457211/1‑44 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:53035/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:649803/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:646614/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:64322/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:639030/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:628247/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:572662/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:571713/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:563133/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:555980/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:551851/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:659332/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:516532/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:516172/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:514864/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:512891/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:45060/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:43740/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:432340/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:424807/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1000720/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:777034/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:980019/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:979104/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:950004/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:945156/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:93162/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:873605/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:832088/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:819446/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:805446/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:778493/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:419749/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:775072/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:741739/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:731509/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:727299/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:695589/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:694223/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:692309/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:679522/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:672665/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:661648/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1200948/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:3992/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1524385/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1513564/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1467107/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1422489/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1344052/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1337602/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1334250/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1277060/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:123332/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1540639/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1199823/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1199713/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1146144/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1129988/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1120573/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1109127/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1099602/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1097226/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1066002/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1021409/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:275126/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:398515/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:36954/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:360500/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:350521/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:34727/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:334745/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:287332/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:284886/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1547513/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:221531/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:199424/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:18022/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1616913/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1612455/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1604212/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1571265/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1565150/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgcg  >  1:1550944/1‑62 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgc   >  1:240834/1‑61 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgc   >  1:801143/1‑61 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGCATTTTcgc   >  1:301424/1‑61 (MQ=255) cGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACAGCAACCACCGAATTTTcgcg  >  1:822743/1‑62 (MQ=255)                                          |                     CGCCATCGCGCTTTCTGGGGGAATAGTGAAGCGTATGGCACTGCAACCACCGCATTTTCGCG  >  W3110S.gb/633198‑633259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑