| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 647,788 | G→T | S336* (TCG→TAG) | citF ← | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 647,788 | 0 | G | T | 96.2% | 66.4 / ‑4.0 | 26 | S336* (TCG→TAG) | citF | citrate lyase, citrate‑ACP transferase (alpha) subunit |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (25/0); total (26/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAG > W3110S.gb/647779‑647840 | cGGGCCAGCTATTGCGCAGCATGTCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:323841/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCa > 1:756926/1‑61 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCa > 1:195482/1‑61 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCa > 1:319255/1‑61 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:890204/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1195693/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:805353/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:772000/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:744789/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:70004/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:598226/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:591432/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:451947/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:442764/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:420505/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:251302/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1623256/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1591151/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1562233/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1424205/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1415313/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1402512/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1349812/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1263617/1‑62 (MQ=255)cGGGCCAGCTATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAg > 1:1217478/1‑62 (MQ=255) aGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGttt > 1:308212/1‑47 (MQ=255) | CGGGCCAGCGATTGCGCAGCATGGCTGTCAAAGCTCTGCACATCCAGCAGTTTGCGGATCAG > W3110S.gb/647779‑647840 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |