Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	49,580	T→G	G604G (GGT→GGG)	kefC →	potassium:proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	49,580	0	T	G	100.0%	201.6 / NA	70	G604G (GGT→GGG)	kefC	potassium:proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (0/70);  total (0/70)

GACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGTAAACA  >  W3110S.gb/49524‑49585                                                         |      gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGATCCGAAGAAGGGAAACa  <  1:801112/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:466657/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:587136/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:585988/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:550167/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:537648/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:526159/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:499222/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:481260/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:683693/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:361262/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:352447/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:351257/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:343910/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1013483/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:339815/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:240772/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:588308/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:342848/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:708298/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:716361/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:723507/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:795388/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:806660/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:808709/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:811792/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:812021/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:850181/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:870583/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:925552/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:947389/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:989076/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:999343/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1393261/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1048419/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:106878/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:115654/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1166147/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1204401/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1232122/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1269661/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:128343/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1291086/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1308673/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1329889/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1345226/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1375824/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1441293/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1450394/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1567811/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:202713/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:198834/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:17838/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1618044/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1583035/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1541212/62‑1 (MQ=255) gACCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:151216/62‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:745713/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:795501/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1505337/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:161155/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:579235/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1138974/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:219783/61‑1 (MQ=255)  aCCGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:15088/61‑1 (MQ=255)   ccGTGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGTAACCGAAGAAGGGAAACa  <  1:1650814/60‑1 (MQ=255)        aaCATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1615298/55‑1 (MQ=255)             cTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:1568472/50‑1 (MQ=255)                    tAATTCAACGTCATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:731318/43‑1 (MQ=38)                           aCGACATGGCTGGCAGGGAACCGAAGAAGGGAAACa  <  1:726149/36‑1 (MQ=255)                                                         |      GACCGCGAACATCTGTCATTAATTCAACGACATGGCTGGCAGGGAACCGAAGAAGGTAAACA  >  W3110S.gb/49524‑49585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑