Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 808,380 G→T intergenic (‑49/+10) ybhB ← / ← bioA predicted kinase inhibitor/7,8‑diaminopelargonic acid synthase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb808,3800GT86.9% 515.8 / 55.7 221intergenic (‑49/+10)ybhB/bioApredicted kinase inhibitor/7,8‑diaminopelargonic acid synthase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base G (0/29);  new base T (192/0);  total (192/29)
Fisher's exact test for biased strand distribution p-value = 6.22e-37
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

CAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGC  >  W3110S.gb/808352‑808422
                            |                                          
cAGAAAGTGTATCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:974464/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCTCGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1193075/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCCCGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:920319/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCg                                   >  1:1121681/1‑38 (MQ=38)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCaaaaaa                     >  1:489127/1‑52 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCTAAAAAATGTTTCATc           >  1:157581/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAAtt                  >  1:614079/1‑54 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCAt            >  1:1377028/1‑61 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCAt            >  1:779634/1‑61 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:477623/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:351694/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:586063/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:569841/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:545246/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:531850/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:509558/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:492157/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:344643/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:39702/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:359000/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:468734/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:437367/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:378246/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:416053/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:408123/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:407474/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:394227/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:397820/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:831300/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:961884/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:956154/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:939372/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:895299/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:889321/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:876553/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:853551/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:85069/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:847425/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:844566/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:646953/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:816778/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:794961/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:764602/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:749097/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:732030/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:728093/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:707296/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:675986/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:655955/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:654078/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1186391/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1413454/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1007009/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1397591/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1386866/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1356936/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1316421/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1303451/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1295784/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1246691/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1228865/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1401251/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:115259/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1143871/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1118260/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:110729/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:110063/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1088542/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1060048/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1048195/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1030655/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1567141/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:171523/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1640777/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1627173/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1454572/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:199363/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1590952/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:207803/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:230269/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1497527/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1469324/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1463303/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:307702/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:1459358/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCGTTATTGGCAAAAAAATGTTTCATc           >  1:332255/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCCCTTATTGGCAAAAAAATGTTTCATc           >  1:428596/1‑62 (MQ=255)
cAGAAAGTGTAGCCAGAAACCCTCACGCTGACTTCCAGTTATTGGCAAAAAAATGTTTCATc           >  1:866692/1‑62 (MQ=255)
         tacccAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:905220/59‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1075637/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1603822/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:190066/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1208100/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1240516/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1250460/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1552133/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1528977/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1518491/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:1408638/62‑1 (MQ=255)
         tAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACcgc  <  1:626617/62‑1 (MQ=255)
                            |                                          
CAGAAAGTGTAGCCAGAAACCCTCACGCGGACTTCTCGTTATTGGCAAAAAAATGTTTCATCCTGTACCGC  >  W3110S.gb/808352‑808422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑