Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	855,978	C→T	G63G (GGG→GGA)	ybiS ←	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	855,978	0	C	T	100.0%	224.7 / NA	79	G63G (GGG→GGA)	ybiS	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (79/0);  total (79/0)

CACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATAC  >  W3110S.gb/855945‑856005                                  |                            cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTGCCATCTGGTACTCGGCGGCAAAATAc  >  1:484769/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTg                     >  1:679048/1‑42 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAAt    >  1:1508570/1‑59 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATa   >  1:995222/1‑60 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATa   >  1:518126/1‑60 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATa   >  1:1649678/1‑60 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATa   >  1:1099377/1‑60 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:409012/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:41314/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:329496/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:473954/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:515722/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:319576/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:242147/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:539308/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:559670/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:559896/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:641551/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:654151/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:313888/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:679851/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:896521/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:964507/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:95668/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:926713/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:926117/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:917929/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:911807/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:911201/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:903900/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:903381/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:707615/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:879947/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:86801/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:835529/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:83430/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:824262/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:796051/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:725816/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:717506/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1213254/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:298380/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:129966/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1286696/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1275100/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1263283/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1241431/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1236669/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1228867/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1378134/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1211733/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1163446/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1141602/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1137098/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1111125/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1051482/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1035714/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1034703/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1501417/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1020869/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:225358/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:218699/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:206196/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:199124/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1641803/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1557818/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1557698/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1524907/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1380244/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1493272/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1431155/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1430532/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTCGGCGGCAAAATAc  >  1:1402521/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:1483753/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:1428752/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:1422255/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:942458/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:810932/1‑61 (MQ=255) cacaCCCGGGTTCGCTTCCATCATATTGGAAAGTCCCATCTGGTACTAGGCGGCAAAATAc  >  1:1374100/1‑61 (MQ=255)                                  |                            CACACCCGGGTTCGCTTCCATCATATTGGAAAGCCCCATCTGGTACTCGGCGGCAAAATAC  >  W3110S.gb/855945‑856005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑