| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 984,674 | A→G | E201E (GAA→GAG) | ycbL → | predicted metal‑binding enzyme |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 984,674 | 0 | A | G | 100.0% | 11.4 / NA | 6 | E201E (GAA→GAG) | ycbL | predicted metal‑binding enzyme |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (6/0); total (6/0) | |||||||||||
TATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGT > W3110S.gb/984669‑984712 | tATGAGCGCCTGCATAATCCCTTCCTGCAAGACGa > 1:1615915/1‑35 (MQ=255)tATGAGCGCCTGCATAATCCCTTCCTGCAAGACGAAAt > 1:307513/1‑38 (MQ=255)tATGAGCGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCg > 1:1464156/1‑43 (MQ=255)tATGAGCGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGt > 1:857945/1‑44 (MQ=255)tATGAGCGCCTGCATAATCCCTTCCAGCAAGACGAAATg > 1:1031126/1‑39 (MQ=38)tATGAGCGCCTGCATAAACCCTTCCTGCAAGACGAAATGCCCg > 1:1091195/1‑43 (MQ=37) | TATGAACGCCTGCATAATCCCTTCCTGCAAGACGAAATGCCCGT > W3110S.gb/984669‑984712 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |