| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 986,889 | G→A | G172G (GGC→GGT) | ompF ← | outer membrane porin 1a (Ia;b;F) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 986,889 | 0 | G | A | 99.6% | 722.3 / NA | 259 | G172G (GGC→GGT) | ompF | outer membrane porin 1a (Ia;b;F) |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (258/0); minor base C (1/0); total (259/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 259 total aligned reads displayed.
TTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTT > W3110S.gb/986858‑986918 | ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAaagaa > 1:414678/1‑52 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAaaga > 1:535206/1‑51 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAaaga > 1:271182/1‑51 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAaaga > 1:200558/1‑51 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAATGAAGTTGGAGtt > 1:813695/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTg > 1:1377337/1‑56 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGTAGtt > 1:986753/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:376114/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:586868/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:582779/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:575159/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:565572/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:557987/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:548122/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1017982/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:504118/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:468274/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:439536/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1022876/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:611166/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:371535/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:339705/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:326404/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:304662/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:293978/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1030041/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:256555/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:245152/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:239583/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1087652/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1006189/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:10016/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:92736/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:918055/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:894204/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:88553/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:870182/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:860176/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:832693/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:822011/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:819997/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:227961/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:783309/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:780982/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:765665/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:762269/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:753325/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:72920/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:689043/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:675329/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:652740/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:640839/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:123021/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1402942/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1073189/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:222341/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1348047/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1331864/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1299257/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1287344/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1278709/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1265742/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:125235/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1360019/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:119900/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1177559/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1172362/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1159350/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1148870/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1131403/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1124288/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1122645/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1096118/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1612102/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:208876/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1045897/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:188773/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:179740/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1641826/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1636869/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:162545/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:161518/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1416403/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1599314/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1529227/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:151133/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1486191/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1471183/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1470180/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1456806/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:1448163/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGt > 1:1574097/1‑60 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGt > 1:1210562/1‑60 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGt > 1:1141359/1‑60 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGAACAAAGAAGTTGGAGtt > 1:1121825/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACAATCAACCAGACCAaagaag > 1:1163315/1‑53 (MQ=18)ttttACCCAGGTACTGAACAGCGAAGTTCAGACAATCAACCAGACCAAAGAAGTTGGAGtt > 1:517919/1‑61 (MQ=255)ttttACCCAGGTACTGAACAGCGAAGTTCAGACAATCAAACAGACCAaagaag > 1:1305253/1‑53 (MQ=18)ttttACCCAGGTACTGAACAGCAAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGAGtt > 1:725888/1‑61 (MQ=255)ttttACACAGGTACTGAACAGCGAAGTTCAGACCATCAACCAGACCAAAGAAGTTGGa > 1:281151/1‑58 (MQ=18) | TTTTACCCAGGTACTGAACAGCGAAGTTCAGGCCATCAACCAGACCAAAGAAGTTGGAGTT > W3110S.gb/986858‑986918 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |