| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 124,558 | 2 bp→CA | coding (1542‑1543/2664 nt) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 124,558 | 0 | T | C | 100.0% | 808.8 / NA | 274 | D514D (GAT→GAC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (274/0); total (274/0) | |||||||||||
| * | W3110S.gb | 124,559 | 0 | C | A | 100.0% | 788.6 / NA | 274 | R515R (CGT→AGG) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (274/0); total (274/0) | |||||||||||
Only 100 of 274 total aligned reads displayed.
AACAAGTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTA > W3110S.gb/124541‑124601 || aaCAAGTGGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1584794/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGatcatc > 1:679414/1‑36 (MQ=25)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGcc > 1:814023/1‑39 (MQ=37)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGACGCGCGTACTTTCGGTa > 1:383597/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:364408/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:513567/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:48860/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:482343/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:465149/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:447800/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:434881/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:413334/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:408099/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:401459/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:367376/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:576006/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:352563/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:331654/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:313574/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:305122/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:288880/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:279203/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:267566/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:264174/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:260427/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:22608/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:800579/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:966579/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:959468/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:932165/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:925456/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:916549/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:902855/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:893282/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:872084/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:858766/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:846132/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:83184/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:548709/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:786846/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:750833/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:733386/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:711051/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:660291/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:643078/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:630735/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:622751/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:605844/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:594271/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:211339/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1138778/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1350786/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1337319/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:131349/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1293134/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:128562/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1265281/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1252417/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1238928/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1223452/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1212122/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1189131/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1164666/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:13536/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1130641/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1119297/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1106808/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1097491/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1096083/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1074164/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:106630/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1037138/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1032100/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1022307/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1003416/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:153816/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:201238/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:184810/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:177033/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:171052/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1642907/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1628721/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1623624/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1612834/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1602138/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1596406/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1583635/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1567132/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1001341/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1523175/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1462618/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1454735/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1434467/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1428270/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1398477/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1384669/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1358195/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACCGATCATCGACGACGAAGCGCGTACTTTCGGTa > 1:557365/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTGGTACAGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1484591/1‑61 (MQ=255)aaCAAGTCGATCAAAGACAGGCTCGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTa > 1:1513764/1‑61 (MQ=255) || AACAAGTCGATCAAAGATCGTCTGGTACCGATCATCGCCGACGAAGCGCGTACTTTCGGTA > W3110S.gb/124541‑124601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |