Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,913,542 2 bp→AT coding (114‑115/1302 nt) rumA ← 23S rRNA (uracil‑5)‑methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,913,5420GA79.1% 504.1 / 113.6 302L39L (CTA→TTA) rumA23S rRNA (uracil‑5)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/63);  new base A (239/0);  total (239/63)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*W3110S.gb2,913,5430CT79.1% 504.1 / 116.1 302T38T (ACG→ACA) rumA23S rRNA (uracil‑5)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/63);  new base T (239/0);  total (239/63)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCC  >  W3110S.gb/2913485‑2913570
                                                         ||                           
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1482395/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1237113/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:724135/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1358336/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1379745/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:58630/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1409639/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:572201/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1476406/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:985343/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:557632/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1506827/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:540126/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:50824/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1000825/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:176284/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:255073/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:337859/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1027769/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:889640/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1098715/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1142543/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:977963/61‑1 (MQ=255)
 aTCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGtt                          <  1:1169411/61‑1 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGttt                         >  1:788673/1‑39 (MQ=37)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGcc        >  1:1581320/1‑56 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCAcac    >  1:1153631/1‑58 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCAc      >  1:1456565/1‑58 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:460025/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:780876/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:907725/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:534196/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:525832/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:939669/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:477042/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:952243/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:329434/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:164380/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:441000/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:4194/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:390437/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:373575/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:620992/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:767376/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:759039/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:747481/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:687233/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:662784/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:652186/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:645787/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:625414/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:548328/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:610432/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:800545/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:815801/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:576418/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:844680/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:857755/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:87210/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1123987/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:131979/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1308525/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1279174/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1262355/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1251716/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1232411/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1208941/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1164499/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1131192/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1324109/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1105447/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1081361/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1074653/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1069111/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1060613/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:10507/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1037441/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1021390/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1004770/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1536408/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:309672/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:291415/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:236663/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:222059/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:196390/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1611526/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1597998/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1589498/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1567968/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1544770/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:316294/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1531558/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1483948/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1440931/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:142047/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1404915/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:1402365/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:138927/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTCGCGCCACAcc   >  1:138569/1‑61 (MQ=255)
                        cgTTTTCCTGCGGCAATAATCCGGGGATAAATAATGTTTTGCCGTTATGTAGCGCCACAcc   >  1:1246522/1‑61 (MQ=255)
                                                         ||                           
TATCTTCAGTAACAGTAACTTCCGCGTTTTCCTGCGGCAATAATCCGGGGATAAATAGCGTTTTGCCGTTATGTCGCGCCACGCCC  >  W3110S.gb/2913485‑2913570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑