| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 3,421,562 | 2 bp→TA | coding (383‑384/1104 nt) | yhdY → | predicted amino‑acid transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,421,562 | 0 | C | T | 100.0% | 54.1 / NA | 21 | A128V (GCC→GTA) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (0/21); total (0/21) | |||||||||||
| * | W3110S.gb | 3,421,563 | 0 | C | A | 95.2% | 52.3 / ‑5.2 | 21 | A128V (GCC→GTA) | yhdY | predicted amino‑acid transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (0/20); total (0/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCTGGAAAATACTCCCGCATCGCGGTCGCTATATTGCGGCCTGGGCGGTGATTTACCCACT > W3110S.gb/3421522‑3421583 || ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTCTGGGCGGTGATTTACCCACt < 1:1374730/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCTGTGATTTACCCACt < 1:362455/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:155262/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:943175/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:770518/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:723570/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:639719/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:490062/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:212276/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:200795/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1572093/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1052310/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1551901/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1506106/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1484456/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1350224/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1268323/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1210871/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1155088/62‑1 (MQ=255)ttCTGGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:1085912/62‑1 (MQ=255)ttCTCGAAAATACTCCCGTATCGCGGTCGCTATATTGCGGTATGGGCGGTGATTTACCCACt < 1:179376/62‑1 (MQ=255) || TTCTGGAAAATACTCCCGCATCGCGGTCGCTATATTGCGGCCTGGGCGGTGATTTACCCACT > W3110S.gb/3421522‑3421583 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |