| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 4,250,996 | 2 bp→CA | coding (621‑622/1116 nt) | malK → | fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,250,996 | 0 | G | C | 100.0% | 17.4 / NA | 13 | L207L (CTG→CTC) | malK | fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (0/13); total (0/13) | |||||||||||
| * | W3110S.gb | 4,250,997 | 0 | G | A | 100.0% | 17.3 / NA | 13 | D208N (GAC→AAC) | malK | fused maltose transport subunit, ATP‑binding component of ABC superfamily and regulatory protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (0/13); total (0/13) | |||||||||||
CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG > W3110S.gb/4250948‑4251009 || ccACGACCAGGTCGAGGCGATGACCCTGGCTGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:688307/62‑1 (MQ=16)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:108632/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1309460/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1427253/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:1604304/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:397813/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:499759/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:629107/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:673187/62‑1 (MQ=12)ccACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:852803/62‑1 (MQ=12) cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:390714/61‑1 (MQ=12) cACGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:806145/61‑1 (MQ=12) aCGACCAGGTCGAGGCGATGACCCTGGCGGACAAGGTCGTGGTGCTCAACGGCGGGcgcg < 1:417434/60‑1 (MQ=12) || CCACGATCAGGTCGAAGCGATGACGCTGGCCGACAAAATCGTGGTGCTGGACGCCGGTCGCG > W3110S.gb/4250948‑4251009 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |