Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 654214–655411 655412 2–1199 31 [0] [0] 32 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 6542130 (0.000)25 (0.830) 21/90 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?W3110S.gb 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

CAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654142‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480
                                                                                                                                            
CAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         >  1:1693446/1‑69
CAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGC                                                                          >  1:2934735/1‑68
   GATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCC                                                                    >  1:1502124/1‑71
     TGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        <  1:735423/65‑1
       ATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 <  1:707422/70‑1
             GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAATCCCCTGAGCG                                                            <  1:2068708/69‑1
                 AACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACC                                                      >  1:959931/1‑71
                   CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATA                                                        <  1:1839944/67‑1
                   CCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGAAGGTGCTAAGCCCCTGAGCGAATACCC                                                     >  1:418805/1‑70
                    CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:351062/62‑1
                    CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAA                                                                       >  1:1531441/1‑51
                    CAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAA                                                                       >  1:192699/1‑51
                     AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAG                                                  >  1:2683350/1‑71
                     AAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAG                                                  <  1:2451145/71‑1
                          AGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCA                                             <  1:2945460/71‑1
                          AGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTG                                                                <  1:2202933/52‑1
                             TAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACC                                          <  1:1526797/71‑1
                             TAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAAT                                                         <  1:1006023/56‑1
                             TAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAAT                                                         <  1:1002366/56‑1
                              AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAAC                                           >  1:48463/1‑69
                               GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCA                                         <  1:2680935/70‑1
                               GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        >  1:1006091/1‑39
                               GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        >  1:2367939/1‑39
                               GCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        >  1:2718928/1‑39
                                     GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                  >  1:2611938/1‑71
                                     GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGA                                                               >  1:1853909/1‑42
                                           AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAG                                                  <  1:1920555/49‑1
                                              CTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:166429/1‑70
                                               TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:521920/1‑69
                                                  GAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCAC                               <  1:582880/61‑1
                                                  GAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAA                                            >  1:3097202/1‑48
                                                   AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGG                                               <  1:2681085/44‑1
                                                     TAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCGAGCGGCAACCAGCAGCATCACCACGTTAG                       <  1:2108780/66‑1
                                                        AGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGC                      <  1:642372/64‑1
                                                         GCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCAT              <  1:2954062/71‑1
                                                              TGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGC          >  1:2152603/1‑70
                                                                   CTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCC    >  1:2427436/1‑71
                                                                     AAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTACCA        >  1:1186628/1‑65
                                                                      AGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  <  1:1322393/70‑1
                                                                                                                                            
CAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654142‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.