| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 121.7 / NA | 35 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (15/20); total (15/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547634‑547758 | cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac < 1:1069798/71‑1 (MQ=255)cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac > 1:601757/1‑71 (MQ=255)cgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAacac < 1:2454480/71‑1 (MQ=255) tCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:1734989/1‑61 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAaca > 1:879913/1‑65 (MQ=255) cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc > 1:1045834/1‑71 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg < 1:1581648/70‑1 (MQ=255) tggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa > 1:583289/1‑51 (MQ=255) gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:827671/71‑1 (MQ=255) tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg < 1:1868315/71‑1 (MQ=255) gCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc > 1:269621/1‑71 (MQ=255) ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc > 1:648151/1‑61 (MQ=255) ccGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTg > 1:1690816/1‑71 (MQ=255) ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaa < 1:952204/37‑1 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:303307/38‑1 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac < 1:2833631/38‑1 (MQ=255) ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg > 1:2919905/1‑70 (MQ=255) tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2104607/56‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGTGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:789661/70‑1 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:257667/55‑1 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:2308276/39‑1 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:662130/39‑1 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg > 1:1289183/1‑41 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg > 1:1266693/1‑41 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATg < 1:171891/70‑1 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg < 1:1106504/62‑1 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa < 1:1735916/50‑1 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:1085703/1‑70 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg > 1:272165/1‑70 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAAGCGCAGGCAGGAGATGAcc > 1:2560012/1‑69 (MQ=255) cAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt < 1:1236899/63‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg > 1:1070457/1‑54 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAgg < 1:507858/58‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa < 1:2717674/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg < 1:1263928/70‑1 (MQ=255) | CGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAG > W3110S.gb/547634‑547758 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |