Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	556,858	A→T	100%	L36Q (CTG→CAG)	folD ←	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	556,858	0	A	T	100.0%	91.7 / NA	30	L36Q (CTG→CAG)	folD	bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (16/14);  total (16/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTT  >  W3110S.gb/556793‑556912                                                                  |                                                       aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt                                                   <  1:495554/71‑1 (MQ=255) aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt                                                   <  1:2816991/71‑1 (MQ=255)  aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                  <  1:2840406/71‑1 (MQ=255)       tGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg                                                  <  1:2376981/66‑1 (MQ=255)            ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg                                        >  1:33316/1‑71 (MQ=255)             tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg                                        <  1:536833/70‑1 (MQ=255)                     aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc                               <  1:2685015/71‑1 (MQ=255)                        tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa                             >  1:1644338/1‑70 (MQ=255)                         aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                           >  1:1859113/1‑71 (MQ=255)                            tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCTATAcgc                        <  1:407521/71‑1 (MQ=255)                                cGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg                                   <  1:2069541/56‑1 (MQ=255)                                  aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAc                          >  1:1789333/1‑63 (MQ=255)                                   tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAAt                            >  1:2803927/1‑60 (MQ=255)                                     cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt               <  1:2304381/71‑1 (MQ=255)                                     cAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt               >  1:250164/1‑71 (MQ=255)                                      aGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                           >  1:270427/1‑58 (MQ=255)                                       gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCTCg                                           <  1:1355738/41‑1 (MQ=38)                                       gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg                                           <  1:2369490/41‑1 (MQ=255)                                            actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg        >  1:599032/1‑71 (MQ=255)                                            actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg        >  1:1506589/1‑71 (MQ=255)                                               acCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCaa     >  1:1019643/1‑71 (MQ=255)                                                cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAAt                            >  1:1639506/1‑47 (MQ=255)                                                cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa                           >  1:2591773/1‑48 (MQ=255)                                                cccACAAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc    >  1:858918/1‑71 (MQ=255)                                                  caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt  >  1:2890937/1‑71 (MQ=255)                                                     caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt  >  1:1469939/1‑68 (MQ=255)                                                          caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc       <  1:2612659/58‑1 (MQ=255)                                                          caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGc       <  1:1855943/58‑1 (MQ=255)                                                           aaCGGCCTGTCCTGGTGACCGCAGTCCGGCTGCAATACGCGCCTGAACttttt           <  1:396329/53‑1 (MQ=255)                                                            aCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg        >  1:3036506/1‑55 (MQ=255)                                                                  |                                                       AAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTT  >  W3110S.gb/556793‑556912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑