| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 140.9 / NA | 46 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (20/26); total (20/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG > W3110S.gb/1303568‑1303691 | aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAAATATGTAAc > 1:117145/1‑71 (MQ=255) acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:360336/71‑1 (MQ=255) acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:2843333/71‑1 (MQ=255) cgaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc > 1:1344713/1‑70 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:1488959/71‑1 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2410270/71‑1 (MQ=255) cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt < 1:2036463/71‑1 (MQ=255) aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:1586330/71‑1 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:523807/64‑1 (MQ=255) tCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:370721/64‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:2251820/60‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt > 1:1324348/1‑71 (MQ=255) cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:2354855/1‑71 (MQ=255) gCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc < 1:1918262/57‑1 (MQ=255) cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt > 1:2211321/1‑67 (MQ=255) gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa < 1:2305927/68‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa < 1:1757031/41‑1 (MQ=38) gACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:708708/1‑70 (MQ=255) aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAc > 1:1068867/1‑68 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg < 1:1623562/67‑1 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt > 1:1860492/1‑53 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt > 1:139244/1‑52 (MQ=255) cTGGAACAACGCGAAAACCGTTATAAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt > 1:935862/1‑69 (MQ=255) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCt < 1:2075642/37‑1 (MQ=37) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:2702354/1‑71 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:3159255/1‑71 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt > 1:825006/1‑52 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt > 1:1535037/1‑52 (MQ=255) acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt < 1:693249/56‑1 (MQ=255) aacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1117558/57‑1 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTg > 1:550270/1‑40 (MQ=38) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTcaaccgcaaccg > 1:2760825/1‑71 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt < 1:2806428/42‑1 (MQ=38) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCaa > 1:916762/1‑61 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:111533/71‑1 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:281434/71‑1 (MQ=255) cgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt < 1:2138288/71‑1 (MQ=255) gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg > 1:968003/1‑69 (MQ=255) aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1379792/49‑1 (MQ=39) aaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1170661/49‑1 (MQ=39) aCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg < 1:3127972/65‑1 (MQ=255) ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:805149/39‑1 (MQ=37) cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTa > 1:999929/1‑65 (MQ=255) gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtgggg > 1:2155235/1‑68 (MQ=255) ttattaACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:2208248/37‑1 (MQ=37) ttaACTATGTAACCTATTTGCCTATTGCTTCTGaa < 1:2877441/35‑1 (MQ=37) | AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTG > W3110S.gb/1303568‑1303691 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |