| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,809,581 | G→A | 44.9% | G52G (GGG→GGA) | mprA → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,809,581 | 0 | G | A | 44.9% | 32.1 / 122.2 | 89 | G52G (GGG→GGA) | mprA | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (24/25); new base A (19/21); total (43/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01 | |||||||||||
CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809514‑2809651 | cTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt < 1:478009/71‑1 (MQ=255) tttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga > 1:1808588/1‑70 (MQ=255) ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgaga > 1:914069/1‑71 (MQ=255) ttGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACgaga > 1:2009959/1‑71 (MQ=255) tgcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc > 1:1872370/1‑71 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:1570517/1‑64 (MQ=255) gcatgcaCATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg > 1:395269/1‑71 (MQ=255) catgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACg < 1:2494605/70‑1 (MQ=255) atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgtt < 1:3022713/71‑1 (MQ=255) atgcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgtt < 1:2934390/71‑1 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:2985557/1‑71 (MQ=255) gcacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACgttgt > 1:926207/1‑71 (MQ=255) cacatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgtt < 1:1920312/71‑1 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACgaga > 1:284716/1‑63 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc > 1:374754/1‑64 (MQ=255) acatgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGttt < 1:2545233/71‑1 (MQ=255) catgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa < 1:761475/71‑1 (MQ=255) catgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTa < 1:1391240/71‑1 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:2384831/1‑71 (MQ=255) atgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt < 1:2953733/71‑1 (MQ=255) tgcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATg > 1:2132706/1‑71 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:2509999/57‑1 (MQ=255) gcaAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:3061625/57‑1 (MQ=255) gcaAAGCAAGCTGCTCGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACga < 1:49745/57‑1 (MQ=255) caAAGCAAGCTGCTGGAGAACCG‑AATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg > 1:2326361/1‑71 (MQ=255) aaaGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg < 1:1550611/71‑1 (MQ=255) agcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGtt < 1:2634924/71‑1 (MQ=255) gcaagcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAtt > 1:1333308/1‑47 (MQ=255) agcTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAtt < 1:132844/71‑1 (MQ=255) gctgctGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTa < 1:3046873/71‑1 (MQ=255) ctgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTAt > 1:1507483/1‑59 (MQ=255) tgctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTAc > 1:2363081/1‑70 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa > 1:676592/1‑41 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt > 1:2599522/1‑65 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAAc < 1:3071864/42‑1 (MQ=255) ctgGAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCt < 1:847054/71‑1 (MQ=255) ggAGAACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg > 1:2699455/1‑70 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttgt > 1:49490/1‑49 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:2118893/69‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:1243266/69‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:2507822/69‑1 (MQ=255) gagaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:1984172/69‑1 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTATATGGCGTTGAATACGCTGGAGt > 1:1369470/1‑71 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATgg < 1:194321/53‑1 (MQ=255) gaACCGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa < 1:2191967/59‑1 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg < 1:2090857/45‑1 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgt < 1:1240430/43‑1 (MQ=255) aaCCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:1537328/59‑1 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg > 1:1473265/1‑43 (MQ=255) ccGCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGtct > 1:274910/1‑70 (MQ=255) cGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTg < 1:343328/63‑1 (MQ=255) gCAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:2883914/1‑71 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg < 1:2524161/40‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACgaga > 1:809496/1‑35 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg < 1:606273/71‑1 (MQ=255) cAATAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAg > 1:1489139/1‑70 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTg < 1:2015007/50‑1 (MQ=255) aTAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGAt < 1:3159960/52‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaa < 1:56254/71‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTTGa < 1:860833/60‑1 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:690665/1‑70 (MQ=255) tAAAATGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa > 1:271701/1‑70 (MQ=255) aaaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa > 1:1064645/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGt > 1:211532/1‑45 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTa > 1:2767830/1‑51 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGa > 1:2748330/1‑58 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaaaa < 1:896858/70‑1 (MQ=255) aaaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAAc > 1:2569138/1‑71 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:58437/1‑48 (MQ=255) aaaTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGa > 1:2530132/1‑48 (MQ=255) aaTGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGa > 1:1013946/1‑47 (MQ=255) aTGCTGAAGGCTCAGGGGATTAACGAGACGTCGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:2099011/71‑1 (MQ=255) aTGCTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca < 1:1164096/71‑1 (MQ=255) tGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACca > 1:179243/1‑70 (MQ=255) gCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTa > 1:1470350/1‑35 (MQ=255) gCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAgg > 1:1494942/1‑62 (MQ=255) cTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTAGGCTGGAg > 1:1278618/1‑54 (MQ=255) cTGAAGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACcaca < 1:2958730/70‑1 (MQ=255) gAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGaa < 1:232726/61‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt < 1:1706293/71‑1 (MQ=255) aaGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAt > 1:2727696/1‑71 (MQ=255) aGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:526329/71‑1 (MQ=255) aGGCTCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTAtt < 1:272969/71‑1 (MQ=255) ggCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTc < 1:1913540/71‑1 (MQ=255) ggCTCAGGGAATTAACGAGACGTTGTTTATGGCGt > 1:2671432/1‑35 (MQ=255) ggCTCAGGGAATTAACGAGACGTTGTTTATGGCGt > 1:2700392/1‑35 (MQ=255) cTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAg < 1:398037/71‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCt < 1:2726676/42‑1 (MQ=255) tCAGGGAATTAACGAGACGTTGTTTATGGCGTTGATTACGCt < 1:2730318/42‑1 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCa > 1:154588/1‑45 (MQ=255) gATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTg < 1:485979/71‑1 (MQ=255) | CTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCGTTGATTACGCTGGAGTCTCAGGAAAACCACAGTATTCAGCCTTCTG > W3110S.gb/2809514‑2809651 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |