| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,740 | T→G | 38.0% | G118G (GGT→GGG) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,740 | 0 | T | G | 38.0% | ‑3.8 / 38.8 | 29 | G118G (GGT→GGG) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (7/11); new base G (4/7); total (11/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01 | |||||||||||
GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 | ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtt < 1:73932/71‑1 (MQ=255)ggTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGt < 1:3097456/70‑1 (MQ=255) tttCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttct > 1:678954/1‑67 (MQ=255) cGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc < 1:898201/70‑1 (MQ=255) gcgcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt > 1:964733/1‑59 (MQ=255) gcCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAgc > 1:1579076/1‑71 (MQ=255) cTACTGGCATGAATGGCTTAACTGGCTGTCAATATTTGCCAATACGGGtttct < 1:1979934/53‑1 (MQ=255) tACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGtttc < 1:2127027/51‑1 (MQ=255) aCTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc > 1:1775635/1‑71 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc < 1:1087982/70‑1 (MQ=255) cTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCAc < 1:1505013/70‑1 (MQ=255) cATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGcc < 1:1673753/71‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGgttct < 1:3014926/44‑1 (MQ=255) tGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGcc < 1:578237/50‑1 (MQ=255) aaTGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGa > 1:1504656/1‑52 (MQ=255) tGGCTTAACTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:867960/71‑1 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGtttcttt > 1:3028982/1‑40 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAgcg > 1:2634671/1‑55 (MQ=255) gCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCa < 1:242451/71‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:934450/70‑1 (MQ=255) aaCTGGCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCg < 1:2983260/70‑1 (MQ=255) aCTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGa < 1:2673929/70‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt > 1:3083745/1‑71 (MQ=255) gCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGt < 1:1000621/71‑1 (MQ=255) gCCGTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGa < 1:2663987/39‑1 (MQ=255) gTCAATATTTGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGAACTGTTTg < 1:1874524/71‑1 (MQ=255) tCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTgg > 1:2377740/1‑71 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTggg > 1:1482686/1‑70 (MQ=255) aaTATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGc < 1:853716/71‑1 (MQ=255) atatTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCa < 1:703406/71‑1 (MQ=255) tttGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAAt > 1:2303009/1‑71 (MQ=255) tttGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCa > 1:866719/1‑38 (MQ=255) tGCCAATACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt > 1:541841/1‑48 (MQ=255) aaTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTc < 1:1199503/45‑1 (MQ=255) aTACGGGGTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAAc > 1:678135/1‑71 (MQ=255) tACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGtt < 1:1990709/54‑1 (MQ=255) ggTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCAttt < 1:1105785/38‑1 (MQ=255) tttctttcGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGc < 1:1250911/71‑1 (MQ=255) | GGTTTACTTTCGTCCGCGCGCCTACTGGCATGAATGGCTTAACTGGCCGTCAATATTTGCCAATACGGGTTTCTTTCGCCCGGATGAAGCGCACCAGCCGCATTTCAGCGACCTGTTTGGGCAAATCATTAACGCCGGGC > W3110S.gb/70671‑70810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |