Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,8311.G100.0% 185.0 / NA 53pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (26/27);  total (26/27)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT  >  W3110S.gb/547761‑547895
                                                                       |                                                                
gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                   <  1:4366623/71‑1 (MQ=255)
  gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                >  1:247472/1‑71 (MQ=255)
   tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                <  1:1720933/70‑1 (MQ=255)
       tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgggggaa                                                           <  1:651312/71‑4 (MQ=255)
        gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAggg                                                               >  1:4399532/1‑66 (MQ=255)
        gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAg                                                                 >  1:1922652/1‑64 (MQ=255)
              aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg                                                         <  1:463194/67‑1 (MQ=255)
              aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACt                                                     >  1:1753331/1‑71 (MQ=255)
               tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                    <  1:4588564/71‑1 (MQ=255)
                     ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc                                              >  1:4048143/1‑71 (MQ=255)
                       cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                            >  1:156673/1‑71 (MQ=255)
                        gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                            <  1:4851636/70‑1 (MQ=255)
                        gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                            <  1:4845574/70‑1 (MQ=255)
                           ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa                                        >  1:4978989/1‑71 (MQ=255)
                            gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc                                       <  1:1643642/71‑1 (MQ=255)
                              tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc                                      <  1:3180089/70‑1 (MQ=255)
                                ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                    <  1:3119815/70‑1 (MQ=255)
                                 gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                <  1:4757686/40‑1 (MQ=255)
                                  cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACt                                                     <  1:61083/51‑1 (MQ=39)
                                   gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                 >  1:2726395/1‑70 (MQ=255)
                                   gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                <  1:1379094/71‑1 (MQ=255)
                                    aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc                                              <  1:303858/56‑1 (MQ=255)
                                     aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt                              >  1:4553718/1‑71 (MQ=255)
                                      aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg                             >  1:2683762/1‑71 (MQ=255)
                                        aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc                           >  1:1305733/1‑71 (MQ=255)
                                         tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa                                                  >  1:4209767/1‑47 (MQ=38)
                                          gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                    <  1:732826/44‑1 (MQ=38)
                                          gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt                         >  1:2158181/1‑71 (MQ=255)
                                           aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc                            >  1:2164174/1‑67 (MQ=255)
                                           aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                        >  1:1364856/1‑71 (MQ=255)
                                            aGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg                       <  1:4451453/71‑1 (MQ=255)
                                             gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                      >  1:3717104/1‑71 (MQ=255)
                                             gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg                      <  1:4464199/71‑1 (MQ=255)
                                               gcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                               >  1:1219564/1‑60 (MQ=255)
                                                cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                   <  1:4901912/71‑1 (MQ=255)
                                                 aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                   <  1:4490697/70‑1 (MQ=255)
                                                 aGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                   >  1:4911308/1‑70 (MQ=255)
                                                   gcgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                >  1:1474482/1‑71 (MQ=255)
                                                   gcgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt                >  1:2487908/1‑71 (MQ=255)
                                                    cgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg               <  1:1915046/71‑1 (MQ=255)
                                                     gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                            >  1:2035341/1‑41 (MQ=38)
                                                     gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa                                        >  1:1636640/1‑45 (MQ=38)
                                                     gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg              >  1:1291173/1‑71 (MQ=255)
                                                      cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGACACCATGTGAATGCCGTCGGGCCa                  <  1:2790484/66‑1 (MQ=255)
                                                       tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                 <  1:3545788/50‑1 (MQ=255)
                                                       tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                <  1:3125652/51‑1 (MQ=255)
                                                       tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc                        <  1:1355105/59‑1 (MQ=255)
                                                         gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                 <  1:4786268/48‑1 (MQ=255)
                                                           aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTTACCATGTGaa                               <  1:4039043/48‑1 (MQ=255)
                                                           aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                 >  1:856227/1‑62 (MQ=255)
                                                            aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt       <  1:3448101/71‑1 (MQ=255)
                                                             tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAAGCCGt                         >  1:1796483/1‑52 (MQ=255)
                                                              aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa                                     <  1:2134099/39‑1 (MQ=38)
                                                               cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc                                      >  1:3045407/1‑37 (MQ=38)
                                                               cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                    >  1:14678/1‑39 (MQ=38)
                                                                tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc            <  1:227309/62‑1 (MQ=255)
                                                                tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc            <  1:1930290/62‑1 (MQ=255)
                                                                tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt       <  1:2900573/67‑1 (MQ=255)
                                                                 ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt  >  1:2492766/1‑71 (MQ=255)
                                                                  gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa                               >  1:4870015/1‑41 (MQ=38)
                                                                       |                                                                
GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT  >  W3110S.gb/547761‑547895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: