Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 185.0 / NA | 53 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (26/27); total (26/27) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT > W3110S.gb/547761‑547895 | gcgtgcgtGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:4366623/71‑1 (MQ=255) gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg > 1:247472/1‑71 (MQ=255) tgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg < 1:1720933/70‑1 (MQ=255) tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gggggaa < 1:651312/71‑4 (MQ=255) gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggg > 1:4399532/1‑66 (MQ=255) gggCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g > 1:1922652/1‑64 (MQ=255) aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg < 1:463194/67‑1 (MQ=255) aTGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACt > 1:1753331/1‑71 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt < 1:4588564/71‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc > 1:4048143/1‑71 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt > 1:156673/1‑71 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt < 1:4851636/70‑1 (MQ=255) gAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt < 1:4845574/70‑1 (MQ=255) ggTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa > 1:4978989/1‑71 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc < 1:1643642/71‑1 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc < 1:3180089/70‑1 (MQ=255) ggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt < 1:3119815/70‑1 (MQ=255) gCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg < 1:4757686/40‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACt < 1:61083/51‑1 (MQ=39) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg > 1:2726395/1‑70 (MQ=255) gAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa < 1:1379094/71‑1 (MQ=255) aaaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc < 1:303858/56‑1 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:4553718/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:2683762/1‑71 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc > 1:1305733/1‑71 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa > 1:4209767/1‑47 (MQ=38) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt < 1:732826/44‑1 (MQ=38) gAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt > 1:2158181/1‑71 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc > 1:2164174/1‑67 (MQ=255) aaGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc > 1:1364856/1‑71 (MQ=255) aGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCg < 1:4451453/71‑1 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg > 1:3717104/1‑71 (MQ=255) gcgcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCgg < 1:4464199/71‑1 (MQ=255) gcAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:1219564/1‑60 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:4901912/71‑1 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:4490697/70‑1 (MQ=255) aGGCGCTGGCAATACTGGAACAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc > 1:4911308/1‑70 (MQ=255) gcgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt > 1:1474482/1‑71 (MQ=255) gcgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt > 1:2487908/1‑71 (MQ=255) cgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATg < 1:1915046/71‑1 (MQ=255) gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt > 1:2035341/1‑41 (MQ=38) gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa > 1:1636640/1‑45 (MQ=38) gcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATgg > 1:1291173/1‑71 (MQ=255) cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGACACCATGTGAATGCCGTCGGGCCa < 1:2790484/66‑1 (MQ=255) tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3545788/50‑1 (MQ=255) tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa < 1:3125652/51‑1 (MQ=255) tGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTc < 1:1355105/59‑1 (MQ=255) gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:4786268/48‑1 (MQ=255) aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTTACCATGTGaa < 1:4039043/48‑1 (MQ=255) aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa > 1:856227/1‑62 (MQ=255) aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:3448101/71‑1 (MQ=255) tACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAAGCCGt > 1:1796483/1‑52 (MQ=255) aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa < 1:2134099/39‑1 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc > 1:3045407/1‑37 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt > 1:14678/1‑39 (MQ=38) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc < 1:227309/62‑1 (MQ=255) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc < 1:1930290/62‑1 (MQ=255) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:2900573/67‑1 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt > 1:2492766/1‑71 (MQ=255) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:4870015/1‑41 (MQ=38) | GCGTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT > W3110S.gb/547761‑547895 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |