New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	W3110S.gb	= 3265510	72 (1.280)	4 (0.080)	3/90	NT	6.1%	coding (374/990 nt)	tdcB	catabolic threonine dehydratase, PLP‑dependent
	?	W3110S.gb	= 3265514	60 (1.210)					coding (370/990 nt)	tdcB	catabolic threonine dehydratase, PLP‑dependent

TAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3265436‑3265510 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcagaaGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAAGGCC  <  W3110S.gb/3265514‑3265445                                                                                                                                                   TAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATG                                                                             >  1:3085398/1‑70    AAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAG                                                                          >  1:580085/1‑70      ATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAA                                                                        >  1:3555164/1‑70          GGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAG                                                                          <  1:384448/64‑1             CTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAG                                                                          <  1:2889254/61‑1                CCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAGTCGTTCTGC                                                              <  1:1288247/70‑1                 CATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAGTCGTTCTGCA                                                             <  1:4238262/70‑1                  ATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGC                                                                            <  1:4414064/54‑1                          AATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATTCAGAA                                                                        >  1:1724785/1‑50                                  TCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAGTCGTTCTGCA                                                             <  1:1520538/53‑1                                    ACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAAGTCGTTCTGCATG                                                           >  1:2711599/1‑53                                       TTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAA                                                                        <  1:3807914/37‑1                                       TTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAA                                                                        <  1:4110957/37‑1                                                                     ctCAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGT                >  1:2066229/3‑63                                                                      GCAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGA       <  1:2616686/71‑1                                                                      GCAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGA       <  1:67502/71‑1                                                                       CAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGG        >  1:3396257/1‑69                                                                       CAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGT                            <  1:4387250/49‑1                                                                       CAGAAGTCGTTCTGCATGGTGATAACTTCAACGACACT                                       >  1:2940727/1‑38                                                                        AGAAGTCGTTCTGCATGGTGATAACTTCAACGACTCTATCGCT                                 <  1:973081/43‑1                                                                        AGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAAG     >  1:4071939/1‑71                                                                        AGAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGT                            <  1:1674266/48‑1                                                                        AGAAGACGTTCTGCATGGTGATAACTTCAACGACACT                                       >  1:4268900/1‑37                                                                         GAAGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAA      <  1:3047621/69‑1                                                                           AGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAAGGCC  >  1:3644985/1‑71                                                                                                                                                   TAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3265436‑3265510 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcagaaGTCGTTCTGCATGGTGATAACTTCAACGACACTATCGCTAAAGTGAGCGAAATTGTCGAAATGGAAGGCC  <  W3110S.gb/3265514‑3265445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.