Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 70,434 C→T 35.3% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb70,4340CT35.3% 97.2 / 83.6 85N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base C (23/32);  new base T (9/21);  total (32/53)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70366‑70504
                                                                    |                                                                      
aaTGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAAc                                                                        <  1:2310256/69‑1 (MQ=255)
       ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtg                                                               <  1:4262801/71‑1 (MQ=255)
       ggAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCt                                                                <  1:2452130/70‑1 (MQ=255)
          gTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                                  <  1:3075099/65‑1 (MQ=255)
          gTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtggtg                                                            >  1:920046/1‑71 (MQ=255)
            aTGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtggt                                                             <  1:2552214/68‑1 (MQ=255)
              gAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCAt                                                                  >  1:1331004/1‑61 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCggg                                                       <  1:4272018/71‑1 (MQ=255)
               aaaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCgg                                                        >  1:177605/1‑70 (MQ=255)
                aaaGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGt                                                      >  1:4087869/1‑71 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:2661369/71‑1 (MQ=255)
                  aGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                    <  1:1052708/71‑1 (MQ=255)
                   gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                    >  1:4214091/1‑70 (MQ=255)
                   gTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTa                                                   <  1:1547293/71‑1 (MQ=255)
                      tGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAAc                                                 <  1:4032806/70‑1 (MQ=255)
                        gCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGc                                                          <  1:2526985/59‑1 (MQ=255)
                        gCTGAAGCGCAAAATGATCCACTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                              >  1:299206/1‑71 (MQ=255)
                          tGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCa                                                                   >  1:1592002/1‑48 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:4849420/1‑71 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:143213/1‑71 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAt                                           <  1:3178533/70‑1 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAt                                           >  1:808161/1‑70 (MQ=255)
                            aaGCGCAAAATGATCCCCTGCTGCAGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          <  1:2244133/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:821225/1‑70 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:3178962/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTTGTGGCGGGTTTAACGCCGATTg                                         <  1:1825813/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:4929717/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:4688932/71‑1 (MQ=255)
                             aGCGCAAAATGATCCCATGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTg                                         <  1:3719451/71‑1 (MQ=255)
                              gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGAtt                                          >  1:952963/1‑69 (MQ=255)
                              gcgcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        <  1:2828115/71‑1 (MQ=255)
                                gcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGttt                                                    <  1:2283843/57‑1 (MQ=255)
                                gcAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAgg                                      >  1:4173430/1‑71 (MQ=255)
                                  aaaaTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGcc                                              <  1:1460786/61‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCtggtgg                                                           >  1:1332301/1‑46 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCaa                                  <  1:4207114/71‑1 (MQ=255)
                                    aaTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCa                                   >  1:3745074/1‑70 (MQ=255)
                                     aTGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGTGTTTAACGCCGATTGAGGCCAAc                                 >  1:349018/1‑71 (MQ=255)
                                     aTGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 >  1:4040682/1‑71 (MQ=255)
                                      tGATCCCCTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACg                                <  1:168008/71‑1 (MQ=255)
                                         tCCCCTGGTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGtt                             <  1:2341774/71‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAATGCCCATCTTGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                           <  1:2648271/71‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGa                                            <  1:1795631/54‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            <  1:4750757/70‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                           <  1:3293732/71‑1 (MQ=255)
                                           cccTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                           >  1:217544/1‑71 (MQ=255)
                                            ccTGCTGCCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAg                                       <  1:69839/58‑1 (MQ=255)
                                             ctgctgCCGGGATACTCGTTTAACGCCCATCTGGTGGCgg                                                        <  1:2743306/40‑1 (MQ=255)
                                              tgctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGttt                                                    <  1:226880/43‑1 (MQ=255)
                                              tgctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                        >  1:5188916/1‑71 (MQ=255)
                                                ctgcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                        <  1:2593691/69‑1 (MQ=255)
                                                ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAtctc                        <  1:383691/69‑1 (MQ=255)
                                                ctgcCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                      >  1:4077908/1‑71 (MQ=255)
                                                 tgcCGGGATACTCGTTTAACGCCCATCTGGTGGCggg                                                       <  1:289747/37‑1 (MQ=255)
                                                  gcCGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 <  1:1751843/58‑1 (MQ=255)
                                                   ccGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttt                   <  1:4407652/71‑1 (MQ=255)
                                                    cGGGATACTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  <  1:1082893/71‑1 (MQ=255)
                                                    cGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTAt                           >  1:433872/1‑62 (MQ=255)
                                                    cGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtttt                  <  1:4740767/71‑1 (MQ=255)
                                                     gggATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                    >  1:3910522/1‑68 (MQ=255)
                                                       gATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               <  1:4670926/71‑1 (MQ=255)
                                                       gATACTCGATTAATGCCCATCTGGGGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttttttt               <  1:4082710/71‑1 (MQ=255)
                                                           cTCGTTTAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:3876599/70‑1 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcg           <  1:3924949/71‑1 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:742901/70‑1 (MQ=255)
                                                           cTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:1526741/70‑1 (MQ=255)
                                                              gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        <  1:1939034/39‑1 (MQ=255)
                                                              gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        <  1:3620652/71‑1 (MQ=255)
                                                              gTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcga          <  1:1711198/69‑1 (MQ=255)
                                                               tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAAc                                 <  1:764957/45‑1 (MQ=255)
                                                               tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAttt                   <  1:3353390/59‑1 (MQ=255)
                                                               tttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        >  1:4668368/1‑70 (MQ=255)
                                                               tttAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGa                                        >  1:4361711/1‑38 (MQ=255)
                                                               tttAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGcc                                    <  1:1125930/42‑1 (MQ=255)
                                                               tttAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATc            <  1:559036/66‑1 (MQ=255)
                                                                ttAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                      >  1:5066175/1‑55 (MQ=255)
                                                                ttAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccga      >  1:4135433/1‑71 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTAt             <  1:1282557/63‑1 (MQ=255)
                                                                 tAATGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:865388/1‑71 (MQ=255)
                                                                 tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTa                            >  1:2699844/1‑48 (MQ=255)
                                                                 tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCg                       >  1:2786567/1‑53 (MQ=255)
                                                                 tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGa                      >  1:3475282/1‑54 (MQ=255)
                                                                 tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGAtt                    >  1:2566997/1‑56 (MQ=255)
                                                                 tAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgaccgac     >  1:3111618/1‑71 (MQ=255)
                                                                   aCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGt                              <  1:1699335/44‑1 (MQ=255)
                                                                   aCGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATcgacc        <  1:450021/66‑1 (MQ=255)
                                                                    cGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGc  >  1:235309/1‑71 (MQ=255)
                                                                    |                                                                      
AATGGTGGGAGTATGAAAAGTATGGCTGAAGCGCAAAATGATCCCCTGCTGCCGGGATACTCGTTTAACGCCCATCTGGTGGCGGGTTTAACGCCGATTGAGGCCAACGGTTATCTCGATTTTTTTATCGACCGACCGC  >  W3110S.gb/70366‑70504

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: