Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,6940AG100.0% 205.9 / NA 59pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/30);  total (29/30)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG  >  W3110S.gb/547628‑547759
                                                                  |                                                                 
tcgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc                                                                >  1:534127/1‑70 (MQ=255)
  gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa                                                              <  1:327262/70‑1 (MQ=255)
    cgcgCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaaaa                                                            >  1:2135312/1‑70 (MQ=255)
     gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAaca                                                          >  1:2722232/1‑71 (MQ=255)
       gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACt                                                        <  1:1232971/71‑1 (MQ=255)
       gcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACt                                                        <  1:396057/71‑1 (MQ=255)
         tCGTCCACACTGGCTGGATGTGCAGCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                      >  1:4887216/1‑71 (MQ=255)
           gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt                                                    <  1:5067175/71‑1 (MQ=255)
              cacaCTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc                                                 >  1:519744/1‑71 (MQ=255)
                cacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc                                               >  1:3320401/1‑71 (MQ=255)
                  ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                             <  1:1980003/71‑1 (MQ=255)
                  ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg                                             >  1:3139252/1‑71 (MQ=255)
                    ggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACCAGGGCAAAACACTGCTTCACGCCggg                                           >  1:160849/1‑71 (MQ=255)
                     gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc                                          >  1:3349349/1‑71 (MQ=255)
                         gATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccg                                        <  1:266681/69‑1 (MQ=255)
                           tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc                                               >  1:4630611/1‑60 (MQ=255)
                           tgtgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                    >  1:4299032/1‑71 (MQ=255)
                             tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGcc                                              <  1:3767398/59‑1 (MQ=255)
                               cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAac                                                           <  1:4201289/44‑1 (MQ=255)
                               cAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcg                                >  1:4112075/1‑71 (MQ=255)
                                aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc                               <  1:3586260/71‑1 (MQ=255)
                                    ggCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg                                  >  1:2250831/1‑64 (MQ=255)
                                     gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                            >  1:2696490/1‑69 (MQ=255)
                                     gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg                            >  1:384454/1‑69 (MQ=255)
                                      cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg                         <  1:3320126/71‑1 (MQ=255)
                                      cttcttCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAg                         <  1:5058816/71‑1 (MQ=255)
                                       ttcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                            <  1:4435995/51‑1 (MQ=255)
                                          ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGc                                                      <  1:4929771/38‑1 (MQ=255)
                                          ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCggg                                           <  1:2210545/49‑1 (MQ=255)
                                           tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga                       <  1:1039481/68‑1 (MQ=255)
                                            cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                            >  1:2236473/1‑46 (MQ=255)
                                            cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt                    <  1:2660972/70‑1 (MQ=255)
                                            cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt                    >  1:3118774/1‑70 (MQ=255)
                                             aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                            <  1:186394/45‑1 (MQ=255)
                                             aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc                                         <  1:3867713/48‑1 (MQ=255)
                                             aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgcc                                         <  1:4890320/48‑1 (MQ=255)
                                              cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                            >  1:993124/1‑44 (MQ=255)
                                              cTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg                                            >  1:2082975/1‑44 (MQ=255)
                                               ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCa                                                  >  1:1168005/1‑37 (MQ=255)
                                                 aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc                               >  1:1021704/1‑54 (MQ=255)
                                                 aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgcgc                               >  1:4108723/1‑54 (MQ=255)
                                                 aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCg               >  1:4323439/1‑70 (MQ=255)
                                                     gCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAccc          <  1:3931543/71‑1 (MQ=255)
                                                       gAACTAAACGAGGGCAAAACACTGCTTCACGCCTGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCAt        <  1:4144220/71‑1 (MQ=255)
                                                       gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAAt                                   >  1:3235176/1‑44 (MQ=255)
                                                       gAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGAccc          <  1:2110361/69‑1 (MQ=255)
                                                         aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg       >  1:4170278/1‑70 (MQ=255)
                                                         aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg       >  1:4793747/1‑70 (MQ=255)
                                                         aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg       >  1:4179074/1‑70 (MQ=255)
                                                         aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGa      <  1:2722538/71‑1 (MQ=255)
                                                          cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa     <  1:4308280/71‑1 (MQ=255)
                                                           tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAc                 <  1:859787/58‑1 (MQ=255)
                                                            aaaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   >  1:953628/1‑71 (MQ=255)
                                                             accGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   <  1:4747890/68‑1 (MQ=255)
                                                             aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAgg  <  1:508565/71‑1 (MQ=255)
                                                             aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   <  1:4823729/70‑1 (MQ=255)
                                                             aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   <  1:372330/70‑1 (MQ=255)
                                                             aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAg   <  1:2995142/70‑1 (MQ=255)
                                                               cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa                                    >  1:344792/1‑35 (MQ=255)
                                                                  |                                                                 
TCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAAGG  >  W3110S.gb/547628‑547759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: