| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 30.3% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 30.3% | 115.5 / 62.7 | 76 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (31/22); new base T (11/12); total (42/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTg > 1:2927250/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGcc > 1:4579884/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGcc > 1:1422556/1‑71 (MQ=255) ttATATTCTCAATCTCACCATTAGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAc > 1:2658730/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCg < 1:1529839/67‑1 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCg > 1:4503386/1‑71 (MQ=255) tatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCgg < 1:4465598/71‑1 (MQ=255) tctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGt > 1:153693/1‑70 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTg > 1:2198243/1‑70 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata > 1:3874231/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGat < 1:1779864/69‑1 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:402743/68‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATTAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:707945/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt < 1:1725919/68‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:2196328/67‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCt < 1:3407310/71‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:1227387/65‑1 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCg < 1:2367782/49‑1 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:1050079/1‑64 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:3575574/71‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:1954964/1‑70 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:4393564/70‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:1993807/71‑1 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTc > 1:815231/1‑36 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt > 1:1220096/1‑36 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:4715124/71‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTATCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1979775/1‑69 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:2791800/55‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:5032734/52‑1 (MQ=255) ggtggtGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:3925517/71‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTG‑‑GTTCCCGc < 1:2179245/59‑1 (MQ=255) gtggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAt > 1:958147/1‑71 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:815770/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:1665045/71‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata < 1:5081785/43‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc < 1:596810/70‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata < 1:2002774/42‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt > 1:5052651/1‑37 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:1329575/1‑59 (MQ=255) gAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:4599295/1‑70 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:4918097/1‑46 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt < 1:2176737/49‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:693924/1‑56 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTcc > 1:5061371/1‑52 (MQ=255) aaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:1993896/1‑39 (MQ=255) aaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt > 1:18370/1‑42 (MQ=255) aaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:642166/1‑71 (MQ=255) aaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:414365/1‑71 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:3373507/1‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:1408842/1‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:4515296/1‑70 (MQ=255) aTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTAc > 1:691296/1‑71 (MQ=255) cAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtc > 1:601553/1‑61 (MQ=255) aGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:4794814/61‑1 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg < 1:2677174/70‑1 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt < 1:4795189/71‑1 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:4983121/1‑57 (MQ=255) cGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTg < 1:2791810/36‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4636555/1‑45 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:3383500/1‑45 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:35705/1‑45 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTccc > 1:4217953/1‑40 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagag > 1:85078/1‑50 (MQ=255) gagaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt < 1:4139535/71‑1 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1792523/42‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc < 1:5143231/71‑1 (MQ=255) gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg < 1:4493000/44‑1 (MQ=255) aTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc < 1:957995/55‑1 (MQ=255) aTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCgg < 1:1351788/71‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:2876777/48‑1 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:3797008/1‑44 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga > 1:2670714/1‑44 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:3111134/1‑54 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:189654/1‑69 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:2774628/1‑69 (MQ=255) tttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:223961/1‑69 (MQ=255) tGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg > 1:1623561/1‑71 (MQ=255) gTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg < 1:3463947/70‑1 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |