| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,587,781 | T→C | 5.8% | F511L (TTT→CTT) | acrD → | aminoglycoside/multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,587,781 | 0 | T | C | 5.8% | 195.2 / 3.7 | 69 | F511L (TTT→CTT) | acrD | aminoglycoside/multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base T (38/27); new base C (2/2); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAA > W3110S.gb/2587712‑2587847 | cTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtt < 1:3151330/71‑1 (MQ=255) aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCttttt > 1:708777/1‑71 (MQ=255) aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCttttt > 1:113821/1‑71 (MQ=255) aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttt < 1:1733117/70‑1 (MQ=255) aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttt < 1:2045502/70‑1 (MQ=255) aCTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttt < 1:3111402/70‑1 (MQ=255) cTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttttt < 1:1905397/71‑1 (MQ=255) tCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTg > 1:3951154/1‑71 (MQ=255) cGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGcc > 1:4828931/1‑71 (MQ=255) cGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGcc < 1:5017457/71‑1 (MQ=255) cGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGc < 1:1798630/70‑1 (MQ=255) ggCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTATGCCt > 1:3924473/1‑71 (MQ=255) gCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTg > 1:3394237/1‑71 (MQ=255) gCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTg > 1:2269036/1‑71 (MQ=255) ctctGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCt > 1:603672/1‑60 (MQ=255) tgtgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTa < 1:295056/70‑1 (MQ=255) tgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTTGTTTAAc < 1:811102/70‑1 (MQ=255) tgCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAAc > 1:1071659/1‑70 (MQ=255) gCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAAc > 1:4633337/1‑69 (MQ=255) ccACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAg < 1:4568354/71‑1 (MQ=255) acacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGAt < 1:4305998/71‑1 (MQ=255) acacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGAt > 1:4659448/1‑71 (MQ=255) acacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGAt < 1:3558190/71‑1 (MQ=255) cacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATg > 1:1033274/1‑71 (MQ=255) cacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATg > 1:898521/1‑71 (MQ=255) cacTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATg < 1:755347/71‑1 (MQ=255) actactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGGTTTTTTGCCTGGTTTAACCAGATGt > 1:2782449/1‑71 (MQ=255) actactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGt > 1:4214645/1‑71 (MQ=255) actactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGt > 1:4208733/1‑71 (MQ=255) ctactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtttttt < 1:1548640/51‑1 (MQ=255) ctactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCttttt < 1:2080586/50‑1 (MQ=255) tactTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGtt > 1:3141591/1‑70 (MQ=255) actTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCtt < 1:2893112/45‑1 (MQ=255) ctTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt < 1:3516230/69‑1 (MQ=255) ctTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt < 1:3618210/69‑1 (MQ=255) ctTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt < 1:61790/69‑1 (MQ=255) aaGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTg > 1:435640/1‑50 (MQ=255) aaGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTg > 1:1815891/1‑50 (MQ=255) gCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTa < 1:34189/53‑1 (MQ=255) gCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGc > 1:2792804/1‑70 (MQ=255) cACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAAc > 1:3654260/1‑71 (MQ=255) cTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACGAGATGTTTAACCGCAACGc > 1:3757809/1‑71 (MQ=255) tGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCa > 1:715317/1‑52 (MQ=255) gAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGcc > 1:4225466/1‑70 (MQ=255) aaaaaaGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCg < 1:4534051/70‑1 (MQ=255) aaaaaaGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCg > 1:1760199/1‑70 (MQ=255) gAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACg > 1:1680047/1‑70 (MQ=255) gAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAAACGCAACGCCGAACGCTACg > 1:4418823/1‑70 (MQ=255) gAGCATCATGGGCAAAAAGGCTTTTTTGACTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACg > 1:4025989/1‑70 (MQ=255) aGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGaa < 1:5009732/71‑1 (MQ=255) aGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGa > 1:3752489/1‑70 (MQ=255) tcatGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt < 1:4908598/43‑1 (MQ=255) tcatGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGttt < 1:1694109/43‑1 (MQ=255) tcatGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAg > 1:691496/1‑71 (MQ=255) tcatGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAg < 1:2079975/71‑1 (MQ=255) catGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATg < 1:5051138/39‑1 (MQ=255) catGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCg < 1:905833/55‑1 (MQ=255) atGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAgg < 1:2062610/70‑1 (MQ=255) atGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAgg < 1:598481/70‑1 (MQ=255) atGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAg > 1:2434722/1‑69 (MQ=255) aggCAAAAAAGCCTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGaaa < 1:3891644/63‑1 (MQ=38) aggCAAAAAAGCCTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGaaa < 1:4942688/63‑1 (MQ=38) aaaaaaGCCTTTTTGCCTGGTTTAACCAGATGTTTAAcc > 1:1258710/1‑39 (MQ=37) aaaaaaGCCTTTTTGCCTGGTTTAACCAGATGTTTAAcc > 1:3680009/1‑39 (MQ=37) aaaaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGaa < 1:2145003/50‑1 (MQ=255) aaaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACg < 1:3313626/44‑1 (MQ=255) aaaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTg > 1:1218310/1‑67 (MQ=255) aaaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCg > 1:1999193/1‑70 (MQ=255) aaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACg > 1:3013108/1‑55 (MQ=255) aaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTg > 1:1708826/1‑66 (MQ=255) aaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaa < 1:2911888/71‑1 (MQ=255) aaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaa < 1:661183/71‑1 (MQ=255) aaaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGa > 1:428385/1‑70 (MQ=255) aaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCa > 1:1792780/1‑39 (MQ=255) aaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGcc > 1:3722287/1‑44 (MQ=255) aaGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaa > 1:4091433/1‑70 (MQ=255) aGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGaaaaa > 1:2253051/1‑58 (MQ=255) aGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGaaaa > 1:408980/1‑71 (MQ=255) gCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGTACGCTACGAAAAAGGGGTGGCg < 1:4360091/65‑1 (MQ=255) gCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGaaa < 1:537365/54‑1 (MQ=255) | CTCACTCCGGCTCTGTGTGCCACACTACTTAAGCCACTGAAAAAAGGTGAGCATCATGGGCAAAAAGGCTTTTTTGCCTGGTTTAACCAGATGTTTAACCGCAACGCCGAACGCTACGAAAAAGGGGTGGCGAAAA > W3110S.gb/2587712‑2587847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |