| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,822,709 | T→A | 6.1% | intergenic (‑52/+169) | dinD ← / ← yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,822,709 | 0 | T | A | 6.1% | 228.3 / 6.1 | 82 | intergenic (‑52/+169) | dinD/yicC | DNA‑damage‑inducible protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (31/46); new base A (3/2); total (34/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TTGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCA > W3110S.gb/3822640‑3822777 | ttGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAtt < 1:1399450/70‑1 (MQ=255) tgatgTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtata > 1:2972687/1‑70 (MQ=255) gttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatat > 1:318321/1‑67 (MQ=255) ttcgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAg < 1:3939998/70‑1 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGtt < 1:3927985/70‑1 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtg > 1:4127320/1‑71 (MQ=255) cgttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtg > 1:4207877/1‑71 (MQ=255) gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:2014833/71‑1 (MQ=255) gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:4282876/71‑1 (MQ=255) gttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:1854831/71‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:379813/58‑1 (MQ=255) ttcATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt > 1:3196697/1‑70 (MQ=255) gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc > 1:769959/1‑71 (MQ=255) gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc > 1:3759083/1‑71 (MQ=255) gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc > 1:4298974/1‑71 (MQ=255) gCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGttaca > 1:4931307/1‑62 (MQ=38) cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtct < 1:2420059/71‑1 (MQ=255) cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtct < 1:2176335/71‑1 (MQ=255) cccACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtc < 1:2815607/70‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:3330602/61‑1 (MQ=255) cACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCa < 1:380158/71‑1 (MQ=255) aCTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCAc < 1:1359706/71‑1 (MQ=255) cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:2553804/47‑1 (MQ=255) cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTAt < 1:4941574/65‑1 (MQ=255) cTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATATAAATACAGtta > 1:3421051/1‑57 (MQ=38) tCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACa > 1:3953569/1‑53 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAg > 1:1869591/1‑59 (MQ=255) cATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGtt < 1:1653698/70‑1 (MQ=255) aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:1064641/43‑1 (MQ=255) aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTAttt < 1:343315/43‑1 (MQ=255) aTGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtct < 1:1420895/71‑1 (MQ=255) tgtgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTtatata > 1:1268833/1‑47 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa < 1:3237464/70‑1 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa < 1:3121878/70‑1 (MQ=255) tgATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa < 1:1598299/70‑1 (MQ=255) gATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgt > 1:1789966/1‑71 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt < 1:2206555/71‑1 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt > 1:3202871/1‑71 (MQ=255) tatCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgtgt < 1:2321082/71‑1 (MQ=255) atCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgt < 1:1114584/48‑1 (MQ=255) tCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCa < 1:4406789/65‑1 (MQ=255) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATAc > 1:1884330/1‑40 (MQ=255) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGt > 1:5139111/1‑50 (MQ=255) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt > 1:2197137/1‑71 (MQ=255) cAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt > 1:2029350/1‑71 (MQ=255) cAATTGCAAAGAAAGTAAATCTGTAACTGTATAtaaat < 1:161974/38‑1 (MQ=37) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:2643253/70‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:147040/70‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:4750845/70‑1 (MQ=255) aaTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCACTGTGCt < 1:1905/70‑1 (MQ=255) aTTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg < 1:2693136/71‑1 (MQ=255) ttGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:4052312/44‑1 (MQ=255) ttGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTtgtg < 1:138747/44‑1 (MQ=255) ttGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTgg > 1:2790653/1‑70 (MQ=255) tGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg > 1:433961/1‑70 (MQ=255) gCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGc > 1:4853247/1‑70 (MQ=255) gCAAAGAAAGTAAATCTGTAACTGTATATAAATAc < 1:4141512/35‑1 (MQ=25) cAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa < 1:2756221/71‑1 (MQ=255) aaagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaa > 1:2935288/1‑70 (MQ=255) aagaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTtct > 1:1708355/1‑54 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:624766/71‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa < 1:123869/71‑1 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaa > 1:4409622/1‑70 (MQ=255) agaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaa > 1:553242/1‑70 (MQ=255) gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa < 1:2876694/71‑1 (MQ=255) gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa < 1:4318295/71‑1 (MQ=255) gaaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaa > 1:259040/1‑70 (MQ=255) aaagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa < 1:2476593/71‑1 (MQ=255) aaagTAAATCTGTAACTGTACTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaaa > 1:1082854/1‑71 (MQ=255) aagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAgt > 1:5113015/1‑54 (MQ=255) aagTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAAc < 1:1448745/71‑1 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:4361878/71‑1 (MQ=255) agTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACa < 1:4735178/71‑1 (MQ=255) gTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACAt < 1:3917765/71‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTAtct < 1:3532729/39‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCa < 1:2701052/61‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCa < 1:4719837/61‑1 (MQ=255) tAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATc < 1:1238805/71‑1 (MQ=255) cTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa > 1:303173/1‑70 (MQ=255) cTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaa > 1:4651628/1‑70 (MQ=255) acaaCTGTATATAAATACAGTTGTGAGTATCTCTCGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAAt > 1:4928947/3‑71 (MQ=38) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCaaa < 1:1192302/68‑1 (MQ=255) tAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATc < 1:3610597/71‑1 (MQ=255) aCTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAg > 1:2409159/1‑40 (MQ=255) cTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTg < 1:971958/46‑1 (MQ=255) gTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTggg > 1:5093385/1‑46 (MQ=255) tATTTATATACAGTTGTGAGTATCTCACGTTCTCAGtgtg < 1:2249243/40‑1 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa < 1:3770157/50‑1 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCaaaaaa < 1:1302493/50‑1 (MQ=255) tttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCa < 1:4848962/70‑1 (MQ=255) ttATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCt < 1:1123590/39‑1 (MQ=255) | TTGATGATGTTCGTTCATTGCCCACTCCATGTGATATCAATTGCAAAGAAAGTAAATCTGTAACTGTATTTATATACAGTTGTGAGTATCTCACGTTCTCAGTGTGCTGGGCAAAAAAACATCGCAAAATCTGGAGCA > W3110S.gb/3822640‑3822777 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |