| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,079 | C→T | 22.8% | R231R (CGC→CGT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,079 | 0 | C | T | 22.8% | 211.3 / 59.4 | 101 | R231R (CGC→CGT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (38/40); new base T (6/17); total (44/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.05e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.93e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT > W3110S.gb/71009‑71149 | cGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGc < 1:4669474/71‑1 (MQ=255) gTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCa < 1:4665277/71‑1 (MQ=255) tCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAt > 1:2913246/1‑71 (MQ=255) tCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAt < 1:4394313/71‑1 (MQ=255) tCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCAt < 1:170059/71‑1 (MQ=255) gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTATCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt < 1:2202498/71‑1 (MQ=255) gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt > 1:3122706/1‑71 (MQ=255) tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc < 1:4512074/71‑1 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCa < 1:2529887/68‑1 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCa < 1:2535870/68‑1 (MQ=255) tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg < 1:2312476/69‑1 (MQ=255) cacaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGc > 1:671234/1‑70 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:1164176/71‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGa < 1:2679162/71‑1 (MQ=255) acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc > 1:2464656/1‑65 (MQ=255) caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGaa < 1:959520/71‑1 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg > 1:3204649/1‑71 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg > 1:2082690/1‑71 (MQ=255) aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg > 1:1848002/1‑71 (MQ=255) tCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg > 1:719957/1‑65 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgct > 1:3120149/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgct > 1:3269972/1‑71 (MQ=255) cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgct > 1:152481/1‑71 (MQ=255) tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgct > 1:3654721/1‑69 (MQ=255) tttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGAGAGGACCAACGCATTAGTCAGGCg < 1:2389893/64‑1 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct > 1:2835798/1‑71 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct > 1:1198496/1‑71 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc < 1:1018312/67‑1 (MQ=255) ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgc < 1:2364299/67‑1 (MQ=255) tCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct < 1:1491298/70‑1 (MQ=255) ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:3201259/70‑1 (MQ=255) ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt < 1:843318/70‑1 (MQ=255) cGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:4173241/71‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCATCGTATCAGCCAGGCGAAGCTGCTTTTg < 1:1339415/71‑1 (MQ=255) gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc < 1:1858114/66‑1 (MQ=255) ccAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg < 1:2889640/56‑1 (MQ=255) cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg < 1:4814126/71‑1 (MQ=255) gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa < 1:3688976/71‑1 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc > 1:744692/1‑71 (MQ=255) cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc < 1:521266/71‑1 (MQ=255) agTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCaa > 1:3205709/1‑69 (MQ=255) ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa > 1:113923/1‑70 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgct > 1:2812950/1‑57 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc > 1:1381761/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc < 1:2506671/70‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCa > 1:2823585/1‑64 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:3038304/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg < 1:1475136/47‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg > 1:2664018/1‑70 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1210610/69‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:1072028/1‑70 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGAACAACGCATTAGTCAGGCGAAgct > 1:2802359/1‑48 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg > 1:60877/1‑45 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTAAGGCGaa > 1:4479193/1‑44 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc < 1:52477/36‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc < 1:2832758/70‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc > 1:3442576/1‑70 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc > 1:2640585/1‑70 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:3555347/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa > 1:4340919/1‑71 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:4349324/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:5009363/71‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg > 1:2033075/1‑36 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgc < 1:500466/42‑1 (MQ=37) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgc < 1:3865251/42‑1 (MQ=37) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAg < 1:2492135/52‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1642497/61‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATc > 1:1959777/1‑71 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATc < 1:4126133/71‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:2156700/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg < 1:3872710/48‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc < 1:4682167/69‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc > 1:2334096/1‑62 (MQ=255) aGCTGGCGCGAGGACCAACGCATTAGTCATGCGAAGCTGCTTTTGAg > 1:3838196/1‑47 (MQ=255) aGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa < 1:1990277/70‑1 (MQ=255) cTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACGCGGATGCCTAt < 1:2616167/63‑1 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCg < 1:4979896/71‑1 (MQ=255) tGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1684754/53‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:3397127/39‑1 (MQ=255) gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCtttt < 1:3403159/39‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg > 1:2820478/1‑71 (MQ=255) gcgAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt < 1:3497401/71‑1 (MQ=255) gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:653168/1‑51 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc > 1:1560542/1‑70 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:1725680/71‑1 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:772709/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:1645925/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:4473757/71‑1 (MQ=255) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:1881769/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAc < 1:2502989/56‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:4416068/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt > 1:1820761/1‑71 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:4686538/71‑1 (MQ=255) aaCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcg > 1:172232/1‑60 (MQ=255) aaCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:3452967/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4957236/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACACGGATGCCTATCGCCACAgtcgg > 1:4346418/1‑60 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:1555022/1‑71 (MQ=255) gCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4780198/1‑69 (MQ=255) cATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:2679282/1‑71 (MQ=255) | CGTCGCGTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT > W3110S.gb/71009‑71149 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |