| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 71,109 | T→C | 25.5% | T241T (ACT→ACC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 71,109 | 0 | T | C | 25.5% | 173.6 / 64.7 | 97 | T241T (ACT→ACC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (30/42); new base C (7/17); total (38/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 103 total aligned reads displayed.
TTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTA > W3110S.gb/71041‑71178 | ttAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTa > 1:113923/1‑70 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAccc > 1:1381761/1‑71 (MQ=255) aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc < 1:2506671/70‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:3038304/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg > 1:2664018/1‑70 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCg < 1:1210610/69‑1 (MQ=255) gATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa < 1:3793083/71‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:1072028/1‑70 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc > 1:3442576/1‑70 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc < 1:2832758/70‑1 (MQ=255) gCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGcc > 1:2640585/1‑70 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:5009363/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa > 1:4340919/1‑71 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:3555347/71‑1 (MQ=255) cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTa < 1:4349324/71‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1642497/61‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATc > 1:1959777/1‑71 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATc < 1:4126133/71‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:2156700/1‑71 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc < 1:4682167/69‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc > 1:2334096/1‑62 (MQ=255) aGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCa < 1:1990277/70‑1 (MQ=255) gCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:2468171/71‑1 (MQ=255) cTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACGCGGATGCCTAt < 1:2616167/63‑1 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCg < 1:4979896/71‑1 (MQ=255) tGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg < 1:1684754/53‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg > 1:2820478/1‑71 (MQ=255) gcgAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt < 1:3497401/71‑1 (MQ=255) gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa > 1:653168/1‑51 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc > 1:1560542/1‑70 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:1725680/71‑1 (MQ=255) ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa < 1:772709/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:4473757/71‑1 (MQ=255) ccAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:1645925/71‑1 (MQ=255) cAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:1881769/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAc < 1:2502989/56‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:4416068/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:4686538/71‑1 (MQ=255) cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt > 1:1820761/1‑71 (MQ=255) aaCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcg > 1:172232/1‑60 (MQ=255) aaCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:3452967/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4957236/1‑71 (MQ=255) aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACACGGATGCCTATCGCCACAgtcgg > 1:4346418/1‑60 (MQ=255) aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:1555022/1‑71 (MQ=255) gCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg > 1:4780198/1‑69 (MQ=255) cATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:2679282/1‑71 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGTtttt < 1:713509/71‑1 (MQ=255) ttAGTCAGGCGAAGCTGCTTTTAAGCACTACCCGGATGCCTATCGCCACCgt < 1:1607947/52‑1 (MQ=255) tAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTAt > 1:1791790/1‑42 (MQ=255) tAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc > 1:3217216/1‑46 (MQ=255) tAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggt > 1:3536565/1‑55 (MQ=255) agccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:798275/4‑71 (MQ=255) agccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:4758692/4‑71 (MQ=255) gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:4425096/68‑1 (MQ=255) gTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:887605/71‑1 (MQ=255) tCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCg > 1:69150/1‑41 (MQ=255) tCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCg > 1:4626014/1‑41 (MQ=255) tCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:832790/71‑1 (MQ=255) ccAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:2714598/70‑1 (MQ=255) cAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt < 1:544581/60‑1 (MQ=255) cAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGttt > 1:3004405/1‑65 (MQ=255) cAGGCGAAGCTGCTTTTGAGCACAACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTgg > 1:122391/1‑62 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg < 1:836815/49‑1 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGttt > 1:863081/1‑64 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:1698685/1‑63 (MQ=255) aGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGa < 1:3361751/70‑1 (MQ=255) aGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa < 1:1320761/55‑1 (MQ=255) ggCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:2641050/64‑1 (MQ=255) ggCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:3877917/71‑1 (MQ=255) ggCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:2943791/71‑1 (MQ=255) gCGAAGCTGTTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCa < 1:818701/71‑1 (MQ=255) gCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCa < 1:614153/71‑1 (MQ=255) gCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:1068693/70‑1 (MQ=255) gAAGCTGCTTTTGAGCACTACCAGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc < 1:2253437/71‑1 (MQ=255) aGCTGCTTTTGAGCACCACCCGGATTCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:4030416/59‑1 (MQ=255) gctgctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActc > 1:2092522/1‑70 (MQ=255) ctgctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:3769388/49‑1 (MQ=255) gctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTg < 1:1916165/56‑1 (MQ=255) ctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt < 1:3561080/71‑1 (MQ=255) ctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:3491189/69‑1 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCgtcggt < 1:4512676/38‑1 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCGTCTGTCGCAATGTTGGTTTTGa < 1:3216711/55‑1 (MQ=255) ttttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa < 1:2452169/55‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:1217900/53‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:5001334/66‑1 (MQ=255) tttGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa < 1:3433345/54‑1 (MQ=255) tttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:2188632/59‑1 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:4457699/1‑60 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc < 1:3916328/69‑1 (MQ=255) tGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTg < 1:172887/51‑1 (MQ=255) tGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcg > 1:4950473/1‑71 (MQ=255) cACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcg > 1:4854600/1‑67 (MQ=255) cTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc > 1:4726753/1‑47 (MQ=255) cTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTa > 1:3169570/1‑71 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt > 1:1172072/1‑41 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:5050550/46‑1 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:1607723/46‑1 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACg < 1:2443458/47‑1 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc < 1:1970935/50‑1 (MQ=255) tACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTT‑ACGATc < 1:1272615/49‑1 (MQ=39) | TTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCGAGTA > W3110S.gb/71041‑71178 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |