Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 2176983–2178179 2178181 3–1199 91 [0] [0] 89 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 21769820 (0.000)72 (1.570) 34/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?W3110S.gb 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT  >  W3110S.gb/2178182‑2178249
                                                                                                                                             
TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                        <  1:1857747/71‑1
 CGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACT                                                                         >  1:348614/1‑69
  GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                       <  1:914073/70‑1
    TATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                      <  1:3851221/69‑1
     ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                   >  1:922806/1‑71
      TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                  <  1:4568621/71‑1
      TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                  <  1:3840277/71‑1
      TCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                 <  1:2296759/71‑1
        ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                 <  1:4389275/70‑1
        ATCATCCGCTAGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                          >  1:1157898/1‑61
           ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                             >  1:4453088/1‑71
           ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                             >  1:4447566/1‑71
           ATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                             >  1:1940198/1‑71
            TCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGC                                                            <  1:519406/71‑1
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                          <  1:513940/71‑1
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                          <  1:4157496/71‑1
              CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                      <  1:4490048/59‑1
                  TGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                       >  1:2980893/1‑70
                    CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     <  1:722939/70‑1
                    CTGAAAATGAACTTTATTTGTTGGCATTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     <  1:3633269/70‑1
                     TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                  <  1:3363217/56‑1
                      GAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                       <  1:103494/66‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                 >  1:4304177/1‑71
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                  >  1:2273171/1‑70
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   <  1:1699197/69‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   <  1:4521898/69‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   <  1:3447505/69‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   <  1:2775434/69‑1
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTAACAATGCG                                                 >  1:1535909/1‑71
                       AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCA                                                                    >  1:2171466/1‑52
                        AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                <  1:177847/71‑1
                        AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                >  1:1111663/1‑71
                         AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                >  1:3047492/1‑70
                          ATGAACTTTATTTGTTGGCCTTAACATATAAATGTCTTACGACTTA                                                                       <  1:1466713/46‑1
                           TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                             <  1:1565453/71‑1
                           TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                             <  1:402455/71‑1
                              ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                          <  1:2014978/71‑1
                              ACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                          <  1:1566172/71‑1
                               CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                          >  1:1273220/1‑70
                               CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGC                                          >  1:3898106/1‑70
                               CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                       <  1:3287961/57‑1
                                TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                       <  1:865338/56‑1
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        <  1:1337809/69‑1
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        >  1:1125819/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        <  1:2994215/69‑1
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        >  1:3085814/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        >  1:2490502/1‑69
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     >  1:353776/1‑56
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     >  1:3903217/1‑56
                                  TATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     >  1:2041855/1‑56
                                      TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                             >  1:105249/1‑44
                                      TGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCG                                                             >  1:2579136/1‑44
                                        TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGC                                >  1:2952496/1‑71
                                        TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                      <  1:4570276/65‑1
                                        TTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                      <  1:1407199/65‑1
                                          GGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTTCTG                              >  1:1610609/1‑71
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                <  1:2618073/49‑1
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                <  1:4554170/49‑1
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                <  1:1338012/49‑1
                                              TTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                       <  1:1167192/42‑1
                                               TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGT                                  >  1:409282/1‑62
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                        >  1:2777852/1‑71
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:1289833/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:4300054/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:579074/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:2543698/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:3971616/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:3190914/70‑1
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   >  1:3515594/1‑44
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                   >  1:3824423/1‑44
                                                AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                     >  1:4420133/1‑42
                                                AACAGAAAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                        >  1:608739/1‑55
                                                 ACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAA                         <  1:254919/69‑1
                                                    GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                    <  1:438327/71‑1
                                                     ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATATCGTGCTGCTCAAGCA                      <  1:4135097/68‑1
                                                     ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTC                           <  1:1279506/63‑1
                                                     ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                      <  1:3808642/52‑1
                                                       AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                 >  1:1027062/1‑71
                                                        AATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                        <  1:516214/63‑1
                                                         ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTG                                 >  1:1104113/1‑53
                                                           GGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTAT                  <  1:387569/66‑1
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT            <  1:1866626/71‑1
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG             <  1:1412087/70‑1
                                                            GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG             <  1:3469304/70‑1
                                                              TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA           <  1:297202/70‑1
                                                              TGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCG              <  1:3988134/67‑1
                                                                ACGACTTATCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG         >  1:4231120/1‑70
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG         >  1:3401679/1‑70
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGG         >  1:2267240/1‑70
                                                                ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCG              <  1:106028/65‑1
                                                                  GACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGTTAGGGAA      <  1:715831/71‑1
                                                                    CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                      >  1:456752/1‑53
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT  >  1:348682/1‑70
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATT    >  1:1869457/1‑68
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                 >  1:4560070/1‑55
                                                                       AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCAT                     <  1:3600803/51‑1
                                                                                                                                             
TCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/2176910‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT  >  W3110S.gb/2178182‑2178249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.