Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 3746913–3748109 3748111 3–1199 81 [0] [0] 82 [tnaB]–[tnaB] [tnaB],insH,[tnaB]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 37469120 (0.000)64 (1.370) 43/94 NT 100% coding (71/447 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 3748112 = 0 (0.000)coding (871/984 nt) tnaB tryptophan transporter of low affinity

TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746840‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  >  W3110S.gb/3748112‑3748181
                                                                                                                                               
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:1458833/1‑70
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           >  1:729073/1‑70
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           <  1:3844331/68‑1
  AAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGT                                                                           <  1:11517/68‑1
   AACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGA                                                                       <  1:2198939/71‑1
    ACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:583530/71‑1
          CCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACAC                                                                 >  1:3222292/1‑70
             CCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              >  1:4000463/1‑70
             CCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              >  1:3505699/1‑70
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGTCAACAGCTAAGTTAGAGAACACCAGCA                                                            <  1:3433916/71‑1
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              >  1:702127/1‑69
              CAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTA                                                                         <  1:3371529/58‑1
                GATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGG                                                          >  1:924553/1‑71
                GATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGG                                                          >  1:4357404/1‑71
                 ATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGA                                                                       <  1:3398042/57‑1
                  TAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGC                                                         >  1:4230450/1‑70
                   AATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCA                                                        >  1:490703/1‑70
                    ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:3353345/70‑1
                    ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:4042832/70‑1
                    ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:2374707/70‑1
                    ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGC                                                             <  1:2285591/64‑1
                    ATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGC                                                             <  1:1015096/64‑1
                      CGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAAC                                                    >  1:1116630/1‑71
                       GAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACT                                                   >  1:2023715/1‑71
                       GAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAA                                                     >  1:120371/1‑69
                       GAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAA                                                     >  1:412669/1‑69
                       GAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAA                                                     >  1:376536/1‑69
                        AACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTC                                                  >  1:4521902/1‑71
                        AACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:2587323/66‑1
                         ACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCG                                                 <  1:1711578/71‑1
                           AACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                               <  1:1189834/71‑1
                             CCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                             <  1:432995/71‑1
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          >  1:1164709/1‑71
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          >  1:1804274/1‑71
                                CAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAA                                                                    >  1:398635/1‑45
                                    GTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGC                                          >  1:908656/1‑67
                                     TGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAA                                                       <  1:4549652/53‑1
                                     TGACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAG                                                                      <  1:1492492/38‑1
                                      GACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACA                                                                  <  1:1292038/41‑1
                                       ACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTG                                   >  1:3701996/1‑71
                                       ACACCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAG                                                              <  1:1262908/44‑1
                                          CCAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTT                                <  1:2530634/71‑1
                                           CAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTG                               >  1:2017307/1‑71
                                           CAAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGC                                                             >  1:2789543/1‑41
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGCACACCAGCA                                                            >  1:637754/1‑41
                                            AAAGAACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCA                                                            >  1:46783/1‑41
                                              AGAACGAACTGGCAACAGCTAAGTTAGAGAACACAAGCAGGCAAAACTC                                                  >  1:4269132/1‑49
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                          >  1:2102796/1‑71
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                          <  1:432391/71‑1
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                          >  1:2016969/1‑71
                                                AACGAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCC                          >  1:2965397/1‑71
                                                   GAACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGAT                                               <  1:3979660/47‑1
                                                    AACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGG                      <  1:1801203/71‑1
                                                     ACTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGG                      >  1:3473944/1‑70
                                                      CTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAA                    <  1:600576/71‑1
                                                      CTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATG                                           <  1:4557929/48‑1
                                                      CTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATG                                           <  1:4552628/48‑1
                                                      CTGGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                             >  1:4627786/1‑46
                                                        GGCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAA                                             >  1:4078923/1‑44
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGA                 <  1:3947771/71‑1
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGA                 >  1:1522836/1‑71
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGA                 >  1:1168428/1‑71
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACG                  <  1:3573972/70‑1
                                                         GCAACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAAC                   <  1:3460423/69‑1
                                                           AACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGA                     >  1:940017/1‑65
                                                           AACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGA                     >  1:682525/1‑65
                                                            ACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTT              <  1:2766752/71‑1
                                                            ACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTT              >  1:197927/1‑71
                                                            ACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAG                       <  1:1413488/62‑1
                                                            ACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTG                                      <  1:4066326/47‑1
                                                            ACAGCTAAGTTAGAGAACACCAGCAGGCAAAACTCG                                                 >  1:657152/1‑36
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCTATAATGCCGTGCTGT                                  <  1:3769103/49‑1
                                                              AGCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCA            >  1:1296139/1‑71
                                                               GCTAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACG                  <  1:2747372/64‑1
                                                                 TAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCAC           >  1:4556088/1‑69
                                                                 TAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTT              >  1:3850824/1‑66
                                                                 TAAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTT              >  1:3232559/1‑66
                                                                  AAGTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCAC           >  1:3091570/1‑68
                                                                    GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCG      <  1:4021328/71‑1
                                                                    GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCG      <  1:1601584/71‑1
                                                                    GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCG      <  1:2439014/71‑1
                                                                    GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCG      <  1:3495392/71‑1
                                                                    GTTAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCG      <  1:4483413/71‑1
                                                                      TAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAA    >  1:1173649/1‑71
                                                                      TAGAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGC       >  1:2516987/1‑68
                                                                        GAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  <  1:173121/71‑1
                                                                                                                                               
TTAAACAGGTCCGCCAGATAATCGAACAACCCCAGCGTGACACCAAAGAACGAACTGGCAACAGCTAAGTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/3746840‑3746912
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagAGAACACCAGCAGGCAAAACTCGATAATGCCGTGCTGTTTGGTGCCGAGGAACGATTTCACCAGCGAATC  >  W3110S.gb/3748112‑3748181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.