| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 556,858 | A→T | 100% | L36Q (CTG→CAG) | folD ← | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 556,858 | 0 | A | T | 100.0% | 123.5 / NA | 42 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (19/23); total (19/23) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACC > W3110S.gb/556790‑556923 | cacaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt > 1:4303259/1‑71 (MQ=255) caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTAGTACCCACCAGCACAACGGCCTGTcc > 1:1446277/1‑71 (MQ=255) caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:513162/71‑1 (MQ=255) caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:2757781/71‑1 (MQ=255) aGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:240546/71‑1 (MQ=255) gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4246107/63‑1 (MQ=255) gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:1927351/63‑1 (MQ=255) cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg < 1:1170792/71‑1 (MQ=255) gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg > 1:1132461/1‑70 (MQ=255) tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:4295924/60‑1 (MQ=255) tttGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:3753185/60‑1 (MQ=255) tttGCTTGCGACATAAATTTGCGATCCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCa < 1:3147308/71‑1 (MQ=255) tgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt > 1:854353/1‑71 (MQ=255) ttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2610477/56‑1 (MQ=255) gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTgg > 1:4631667/1‑56 (MQ=255) gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg < 1:2203088/70‑1 (MQ=255) gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:2412718/1‑71 (MQ=255) aCATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3072522/71‑1 (MQ=255) cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:234053/1‑70 (MQ=255) aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg < 1:1275943/50‑1 (MQ=255) aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaa < 1:2305519/71‑1 (MQ=255) aaaTTTGCGATGCATGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa > 1:3690934/1‑71 (MQ=255) aaaTTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGt < 1:22627/44‑1 (MQ=255) tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:3735782/71‑1 (MQ=255) tGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACgcgc > 1:2324632/1‑71 (MQ=255) aGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtt < 1:1206503/71‑1 (MQ=255) gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:4016842/71‑1 (MQ=255) ggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATa > 1:1042736/1‑56 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:1722570/71‑1 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:1630720/1‑71 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:438645/71‑1 (MQ=255) ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg > 1:2810348/1‑46 (MQ=255) ccaccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACt < 1:3565894/71‑1 (MQ=255) caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt < 1:3052522/71‑1 (MQ=255) ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt < 1:4647163/59‑1 (MQ=255) aGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa > 1:627340/1‑69 (MQ=255) cacaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAg > 1:3880659/1‑59 (MQ=255) caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:2057170/1‑70 (MQ=255) caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:4249319/1‑70 (MQ=255) aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCaaa > 1:3933453/1‑35 (MQ=39) aaCGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCa > 1:3684223/1‑71 (MQ=255) ggCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCAcc > 1:4614953/1‑70 (MQ=255) | CACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCGCACC > W3110S.gb/556790‑556923 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |