| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 41.0% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 41.0% | 41.9 / 72.6 | 61 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (24/12); new base T (13/12); total (37/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
AAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAG > W3110S.gb/70514‑70647 | aaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGc > 1:4232607/1‑71 (MQ=255) aGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGcc > 1:1912352/1‑71 (MQ=255) ggTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCt < 1:494026/67‑1 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:1999385/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGa > 1:3075074/1‑68 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCg > 1:4002579/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCg > 1:3648492/1‑71 (MQ=255) tatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCgg > 1:65406/1‑71 (MQ=255) tatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCg < 1:2709883/70‑1 (MQ=255) ttCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:2018826/1‑61 (MQ=255) tctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGt > 1:3764322/1‑70 (MQ=255) ctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGa > 1:1834618/1‑71 (MQ=255) ctcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt > 1:4172449/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:78619/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:1460316/1‑71 (MQ=255) tcACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:2567922/1‑71 (MQ=255) cACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc > 1:4448574/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:3885100/1‑71 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAttt < 1:2545916/67‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTg > 1:2470306/1‑71 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTc > 1:2922449/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:2782514/1‑71 (MQ=255) gcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCg < 1:4530415/71‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:3233533/71‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGata > 1:4097260/1‑54 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGa < 1:3046507/43‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:4329723/1‑71 (MQ=255) gcAGGGGGTGGGGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata < 1:2951537/52‑1 (MQ=39) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:3948649/71‑1 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTg > 1:1490332/1‑56 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:2451252/1‑69 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:2125186/54‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2220800/71‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:4713743/1‑69 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt < 1:2759515/71‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:3166044/44‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:1009540/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:1132433/48‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:4445163/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGATATtcatca < 1:3092981/71‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1174818/1‑71 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGtt < 1:2619438/48‑1 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:815298/53‑1 (MQ=255) aaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTc > 1:1028808/1‑48 (MQ=255) aGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt > 1:62753/1‑71 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcg > 1:3767000/1‑71 (MQ=255) gACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:1683277/45‑1 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:932821/1‑70 (MQ=255) aCGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcgt > 1:770123/1‑70 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:2942242/49‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:1760108/1‑58 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:932485/1‑71 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATc > 1:1399277/1‑71 (MQ=255) gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATcc < 1:4449213/71‑1 (MQ=255) gaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATcc > 1:541159/1‑71 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg < 1:1350868/71‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg < 1:3322878/71‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1866819/40‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc < 1:4248309/56‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:286748/1‑40 (MQ=255) ttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCg > 1:3548519/1‑35 (MQ=255) ttGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc > 1:2090079/1‑36 (MQ=255) tCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAg > 1:3516586/1‑68 (MQ=255) | AAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAG > W3110S.gb/70514‑70647 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |