Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,303,6330GT100.0% 205.3 / NA 67Q246Y (CAG→TAToppAoligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/33);  total (34/33)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303566‑1303695
                                                                   |                                                              
tAAACGAACTAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                             <  1:3280780/71‑1 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:3289469/1‑71 (MQ=255)
  aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc                                                           >  1:4148953/1‑71 (MQ=255)
   acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc                                                          <  1:3145027/71‑1 (MQ=255)
     gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:4650862/71‑1 (MQ=255)
      aacgaaTCGTTCTTGAACGCAGACCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt                                                       <  1:4047223/71‑1 (MQ=255)
       acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt                                                      >  1:1790086/1‑71 (MQ=255)
        cgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAttt                                                     >  1:2502540/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg                                                    >  1:4148353/1‑63 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:3272204/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:90820/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:1420009/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:2667342/1‑71 (MQ=255)
                 cTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:82032/1‑71 (MQ=255)
                  ttGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:10007/58‑1 (MQ=255)
                   tGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                           >  1:2655090/1‑70 (MQ=255)
                    gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTc                                         >  1:1726304/1‑71 (MQ=255)
                     aaCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        <  1:4656581/71‑1 (MQ=255)
                      aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       <  1:3110521/71‑1 (MQ=255)
                       cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       >  1:1433736/1‑70 (MQ=255)
                          aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt                                   <  1:3953352/71‑1 (MQ=255)
                           gCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                  <  1:1479425/71‑1 (MQ=255)
                                aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             <  1:1319175/71‑1 (MQ=255)
                                 ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCt                                                 <  1:1805110/50‑1 (MQ=255)
                                  cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                           >  1:3866344/1‑71 (MQ=255)
                                  cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                           <  1:3733464/71‑1 (MQ=255)
                                  cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg                           >  1:177796/1‑71 (MQ=255)
                                   tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa                                                        <  1:1554031/41‑1 (MQ=38)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:2736821/46‑1 (MQ=39)
                                    aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                  <  1:239895/46‑1 (MQ=39)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc                                                   >  1:3044982/1‑44 (MQ=38)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:4118254/1‑66 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:3442340/1‑66 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                          <  1:354938/69‑1 (MQ=255)
                                     cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTc                         <  1:3465417/70‑1 (MQ=255)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       <  1:3058903/55‑1 (MQ=255)
                                      tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt                          <  1:979758/68‑1 (MQ=255)
                                       ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:2707349/1‑71 (MQ=255)
                                       ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:3052442/1‑71 (MQ=255)
                                       ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:1075057/1‑71 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg                                       <  1:3438123/53‑1 (MQ=255)
                                        gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAcc                     >  1:987819/1‑71 (MQ=255)
                                         aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCg                    >  1:1682321/1‑71 (MQ=255)
                                          acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                            >  1:2887724/1‑46 (MQ=39)
                                          acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGc                   <  1:3737881/71‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        <  1:4470897/47‑1 (MQ=39)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGaa                                     <  1:1447104/50‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGtt                                  <  1:1023000/53‑1 (MQ=255)
                                             acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:1983644/1‑58 (MQ=255)
                                              cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc               >  1:1180562/1‑71 (MQ=255)
                                              cgcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTAcc               >  1:4502657/1‑71 (MQ=255)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:156331/1‑56 (MQ=255)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa                             >  1:2324500/1‑56 (MQ=255)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCACCCGCTACCg              <  1:570683/71‑1 (MQ=255)
                                               gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg              <  1:4018495/71‑1 (MQ=255)
                                                 gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                              <  1:4136210/53‑1 (MQ=255)
                                                  aaaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAg           <  1:3817516/71‑1 (MQ=255)
                                                   aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgt          >  1:2456020/1‑71 (MQ=255)
                                                      ccGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt       <  1:3086371/71‑1 (MQ=255)
                                                       cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt                                        <  1:1035655/37‑1 (MQ=37)
                                                       cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGt             <  1:3939166/64‑1 (MQ=255)
                                                         ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACGGTAgtg         <  1:2595380/66‑1 (MQ=255)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa   <  1:2582165/70‑1 (MQ=255)
                                                           attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  >  1:2981697/1‑71 (MQ=255)
                                                             taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                              >  1:499767/1‑41 (MQ=38)
                                                             taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                      >  1:787876/1‑49 (MQ=39)
                                                             taACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt  >  1:2315778/1‑69 (MQ=255)
                                                                   |                                                              
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT  >  W3110S.gb/1303566‑1303695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: