| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,916,448 | C→T | 11.1% | R28H (CGT→CAT) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,916,448 | 0 | C | T | 11.1% | 131.9 / 7.2 | 45 | R28H (CGT→CAT) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base T (3/2); total (25/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGC > W3110S.gb/1916380‑1916513 | gcgACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCAcgc < 1:4268299/71‑1 (MQ=255) aCGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTg < 1:539828/71‑1 (MQ=255) gTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGa > 1:4268015/1‑70 (MQ=255) ttACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCAc < 1:3082019/63‑1 (MQ=255) aCGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATAtc > 1:2804523/1‑71 (MQ=255) aCGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATAtc > 1:690994/1‑71 (MQ=255) cgcgCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATAtc < 1:114013/70‑1 (MQ=255) cTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTa < 1:3431725/71‑1 (MQ=255) tCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTa > 1:2917702/1‑70 (MQ=255) cACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTa > 1:4694234/1‑69 (MQ=255) aCTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCATGCGTGATATCTTCTACAg > 1:3263003/1‑71 (MQ=255) aCTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCATGCGTGATATCTTCTACAg > 1:1347619/1‑71 (MQ=255) aCTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCATGCGTGATATCTTCTACAg > 1:3410728/1‑71 (MQ=255) cTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGc > 1:299918/1‑71 (MQ=255) aCCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCg > 1:1811919/1‑69 (MQ=255) aCCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCg < 1:2582263/69‑1 (MQ=255) aCCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCg > 1:2752898/1‑69 (MQ=255) aCCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGa < 1:1724857/70‑1 (MQ=255) cGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTa > 1:1521145/1‑62 (MQ=255) tgctgcGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGa > 1:257289/1‑46 (MQ=255) gctgcGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTg < 1:3562521/67‑1 (MQ=255) gcGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGatat > 1:4276467/1‑45 (MQ=255) gcGTCTCTTCCTTTAATACAGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCt > 1:720354/1‑63 (MQ=255) gTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTAt < 1:705524/71‑1 (MQ=255) ctctTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTAt > 1:210797/1‑69 (MQ=255) ttCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGc < 1:2931003/69‑1 (MQ=255) ccTTTAATACCGGAATTTGATCAGCATGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCa < 1:82415/68‑1 (MQ=255) ccTTTAATACCGGAATTTGATCAGCATGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCa < 1:765251/68‑1 (MQ=255) ccTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAg < 1:3498809/70‑1 (MQ=255) ccTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAg < 1:2938355/70‑1 (MQ=255) cTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc < 1:3369005/70‑1 (MQ=255) cTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc < 1:2728246/70‑1 (MQ=255) cTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc < 1:2722589/70‑1 (MQ=255) tAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTAt > 1:1917493/1‑60 (MQ=255) tAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGc > 1:2725926/1‑71 (MQ=255) ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAg > 1:580685/1‑41 (MQ=255) ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCa > 1:326834/1‑63 (MQ=255) ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCt < 1:3541874/71‑1 (MQ=255) ccGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCt < 1:2422159/71‑1 (MQ=255) tttGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc < 1:3874581/56‑1 (MQ=255) ttGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGc < 1:3815322/55‑1 (MQ=255) tGATCAGCACGCGTGATGTCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCgg > 1:1907795/1‑70 (MQ=255) gATCAGCACGCGTGATATCTTCTACAGCGAAGGTCt > 1:929541/1‑36 (MQ=255) tCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTaa < 1:1484893/70‑1 (MQ=255) tCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAg > 1:1160670/1‑71 (MQ=255) cAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTAtt > 1:2135048/1‑44 (MQ=255) cAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGc > 1:649901/1‑71 (MQ=255) | GCGACGTTACGCGCTCACTTACCGTCGCATGCTGCGTCTCTTCCTTTAATACCGGAATTTGATCAGCACGCGTGATATCTTCTACAGCGAAGGTCTGGCCTGCTATTGCAAGCAGGCCAGCTAACGCGGTAAGC > W3110S.gb/1916380‑1916513 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |