| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,514 | T→C | 100% | pseudogene (647/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,514 | 0 | T | C | 100.0% | 195.9 / NA | 55 | pseudogene (647/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/33); total (22/33) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCA > W3110S.gb/3824447‑3824577 | gACGGGCGCATCATTGAAGTGCAGGTGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCtt > 1:19444/1‑71 (MQ=255) gggCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt > 1:1155883/1‑71 (MQ=255) gcgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:4113949/67‑1 (MQ=255) cgcATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttg < 1:1515192/66‑1 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCCCCCATGAAGAGCTACTCCCCTTGTTGGCGCTGGc > 1:1465924/1‑71 (MQ=255) atTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGc > 1:1709945/1‑71 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCtt < 1:1738271/58‑1 (MQ=255) ttGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGcc < 1:3131606/71‑1 (MQ=255) tGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGcc < 1:2354812/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:4417783/1‑70 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:1029565/1‑70 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:395542/1‑70 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:806098/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:567389/1‑70 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc > 1:4123170/1‑70 (MQ=255) aaGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:4523435/70‑1 (MQ=255) cAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑gggg < 1:2243050/70‑1 (MQ=255) aGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑ggggga < 1:325776/71‑3 (MQ=255) gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcg < 1:2437694/71‑1 (MQ=255) gCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGACCCGAGGGGg‑aatcgaatc < 1:4499115/71‑1 (MQ=255) cAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcgaatc > 1:2640708/1‑70 (MQ=255) aGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa > 1:1454572/1‑71 (MQ=255) aGAAGGCGAGCAGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa > 1:3344862/1‑71 (MQ=255) aGAAGGCGAGCAGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa > 1:3339346/1‑71 (MQ=255) gAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:296282/52‑1 (MQ=255) aaGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCTAATCCAtt > 1:1623665/1‑71 (MQ=255) aGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTg < 1:2896200/71‑1 (MQ=255) aGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCg < 1:833556/71‑1 (MQ=255) gCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGa > 1:2021230/1‑71 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:479488/40‑1 (MQ=38) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGt < 1:3903044/62‑1 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGc < 1:3579384/65‑1 (MQ=255) ttCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑ggggga < 1:2668799/48‑3 (MQ=255) tCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACg > 1:1014895/1‑68 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg > 1:2363465/1‑70 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg < 1:1253262/70‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAg < 1:3662907/70‑1 (MQ=255) aCCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGaa < 1:1395267/71‑1 (MQ=255) cATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggc > 1:3968211/1‑71 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa > 1:4114454/1‑50 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa > 1:712740/1‑50 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTg < 1:3738464/53‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGc < 1:382667/57‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggc < 1:1861839/69‑1 (MQ=255) gAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcgaat < 1:2683285/46‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCATAAGgcggcg > 1:3084796/1‑71 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc < 1:1469392/70‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcggc < 1:3730553/70‑1 (MQ=255) aaGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAggcg < 1:4199753/68‑1 (MQ=255) agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGgcgc < 1:2303595/71‑1 (MQ=255) agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGgcgc < 1:1183897/71‑1 (MQ=255) gagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCt < 1:1566880/71‑1 (MQ=255) aCTCACCTTGTTGGCGCTGGCCCGA‑GGGGTAATCGAATCCATTGTAGCGACg > 1:3708060/1‑52 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa < 1:1455440/69‑1 (MQ=255) cTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCa < 1:642195/69‑1 (MQ=255) | GACGGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGCTGGCA > W3110S.gb/3824447‑3824577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |