BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	4,563,589	T→A	100%	K207M (AAG→ATG)	iadA ←	isoaspartyl dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	4,563,589	0	T	A	100.0%	194.9 / NA	63	K207M (AAG→ATG)	iadA	isoaspartyl dipeptidase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (31/32); total (31/32)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCG  >  W3110S.gb/4563522‑4563653

ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                               >  1:2870892/1‑71 (MQ=255)

 gTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                              <  1:3542389/71‑1 (MQ=255)

  tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatttt                                                              <  1:3143918/70‑1 (MQ=255)

  tCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                             <  1:1097177/71‑1 (MQ=255)

    ggCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                           <  1:4720765/71‑1 (MQ=255)

     gcagcaGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTg                                                          <  1:2751859/71‑1 (MQ=255)

       agcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                             >  1:4711187/1‑66 (MQ=255)

         cagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                      >  1:3194845/1‑71 (MQ=255)

          agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                     <  1:2236127/71‑1 (MQ=255)

          agcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                     <  1:294833/71‑1 (MQ=255)

           gcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                     <  1:4437145/70‑1 (MQ=255)

            cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   <  1:3301512/71‑1 (MQ=255)

            cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   <  1:2095070/71‑1 (MQ=255)

            cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   <  1:2100538/71‑1 (MQ=255)

             ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt                                                               >  1:1997275/1‑58 (MQ=255)

             ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   >  1:3045384/1‑70 (MQ=255)

             ttGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   >  1:2529096/1‑70 (MQ=255)

              tGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                 >  1:1797382/1‑71 (MQ=255)

               gCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGt                                                         >  1:2652662/1‑62 (MQ=255)

                cTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                  <  1:3700851/68‑1 (MQ=255)

                   aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                            >  1:3932867/1‑71 (MQ=255)

                   aTCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                            >  1:4541582/1‑71 (MQ=255)

                      ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac                                         <  1:920797/71‑1 (MQ=255)

                      ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca                                          <  1:828499/70‑1 (MQ=255)

                       gCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCatt                                                                >  1:3042708/1‑47 (MQ=255)

                        cacaTCGAAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc                                                    <  1:2647167/58‑1 (MQ=255)

                         acaTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGTAACACGGt                                      <  1:3175852/71‑1 (MQ=255)

                          caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                  >  1:1467813/1‑58 (MQ=255)

                          caTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAt                                                  >  1:2411639/1‑58 (MQ=255)

                           aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                     <  1:1401728/54‑1 (MQ=255)

                           aTCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGcc                                                     <  1:865219/54‑1 (MQ=255)

                              gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa                                                             >  1:671364/1‑43 (MQ=255)

                              gCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                 >  1:4375613/1‑71 (MQ=255)

                               cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                <  1:3858141/71‑1 (MQ=255)

                               cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGc                                 <  1:1771761/70‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa                                                   <  1:2217359/50‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACgg                                       <  1:3267702/62‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa                               <  1:4378138/70‑1 (MQ=255)

                                 gTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg                              >  1:2336826/1‑71 (MQ=255)

                                  ttttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAgg                             <  1:4635578/71‑1 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACt                                                           >  1:2215004/1‑39 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                >  1:1357840/1‑66 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                           >  1:3222839/1‑71 (MQ=255)

                                    ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt                           >  1:2648178/1‑71 (MQ=255)

                                     tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                    >  1:3228357/1‑61 (MQ=255)

                                     tCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                    >  1:2192324/1‑61 (MQ=255)

                                      ccAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                          <  1:2722704/70‑1 (MQ=255)

                                       cagcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTa                         <  1:140010/70‑1 (MQ=255)

                                         gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGccc                                                    >  1:3376983/1‑41 (MQ=255)

                                         gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg                      <  1:3088890/71‑1 (MQ=255)

                                         gcagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccg                      >  1:2436494/1‑71 (MQ=255)

                                          cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                        >  1:2012242/1‑68 (MQ=255)

                                          cagGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                        >  1:420970/1‑68 (MQ=255)

                                           agGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtg                                                 >  1:2371879/1‑42 (MQ=255)

                                             gTCATAAATAGGCTGTAACGCCATTTTACTGTCGc                                                      <  1:178168/35‑1 (MQ=255)

                                               cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa                                            >  1:1087685/1‑43 (MQ=255)

                                                aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGttt                          <  1:1784348/60‑1 (MQ=255)

                                                aTAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGc                >  1:443166/1‑70 (MQ=255)

                                                   aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGGGACGCCAGGTTTACCGCCGAGCaaa             <  1:3338351/70‑1 (MQ=255)

                                                     tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGACCACGGTGACGc                                 <  1:1703652/48‑1 (MQ=39)

                                                          tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAc                        <  1:3958063/52‑1 (MQ=255)

                                                          tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc     >  1:3632728/1‑71 (MQ=255)

                                                          tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc     >  1:3313789/1‑71 (MQ=255)

                                                          tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc     >  1:2744691/1‑71 (MQ=255)

                                                           gTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTAccgccg                   <  1:4199227/56‑1 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGa                                    >  1:4528932/1‑38 (MQ=255)

                                                            tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcc                                <  1:4134007/42‑1 (MQ=255)

                                                             aaCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAcgcg  >  1:340149/1‑71 (MQ=255)

GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCG  >  W3110S.gb/4563522‑4563653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`